Isgrig Kevin, Chien Wade W
National Institute on Deafness and Other Communication Disorders, National Institutes of Health.
National Institute on Deafness and Other Communication Disorders, National Institutes of Health; Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins School of Medicine;
J Vis Exp. 2018 Mar 2(133):56648. doi: 10.3791/56648.
Inner ear gene therapy offers great promise as a potential treatment for hearing loss and dizziness. One of the critical determinants of the success of inner ear gene therapy is to find a delivery method which results in consistent transduction efficiency of targeted cell types while minimizing hearing loss. In this study, we describe the posterior semicircular canal approach as a viable method for inner ear gene delivery in neonatal mice. We show that gene delivery through the posterior semicircular canal is able to perfuse the entire inner ear. The easy anatomic identification of the posterior semicircular canal, as well as minimal manipulation of the temporal bone required, make this surgical approach an attractive option for inner ear gene delivery.
内耳基因治疗作为一种治疗听力损失和头晕的潜在方法具有巨大前景。内耳基因治疗成功的关键决定因素之一是找到一种递送方法，该方法能使靶向细胞类型的转导效率保持一致，同时将听力损失降至最低。在本研究中，我们描述了后半规管途径作为新生小鼠内耳基因递送的一种可行方法。我们表明，通过后半规管进行基因递送能够灌注整个内耳。后半规管易于解剖识别，且所需的颞骨操作极少，这使得这种手术方法成为内耳基因递送的一个有吸引力的选择。
