Guo Jing-Ying, He Lu, Qu Teng-Fei, Liu Yu-Ying, Liu Ke, Wang Guo-Peng, Gong Shu-Sheng
Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University.
Department of Otolaryngology-Head and Neck Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University.
J Vis Exp. 2018 May 25(135):57351. doi: 10.3791/57351.
Local delivery of therapeutic drugs into the inner ear is a promising therapy for inner ear diseases. Injection through semicircular canals (canalostomy) has been shown to be a useful approach to local drug delivery into the inner ear. The goal of this article is to describe, in detail, the surgical techniques involved in canalostomy in both adult and neonatal mice. As indicated by fast-green dye and adeno-associated virus serotype 8 with the green fluorescent protein gene, the canalostomy facilitated broad distribution of injected reagents in the cochlea and vestibular end-organs with minimal damage to hearing and vestibular function. The surgery was successfully implemented in both adult and neonatal mice; indeed, multiple surgeries could be performed if required. In conclusion, canalostomy is an effective and safe approach to drug delivery into the inner ears of adult and neonatal mice and may be used to treat human inner ear diseases in the future.
将治疗性药物局部递送至内耳是一种治疗内耳疾病的有前景的疗法。通过半规管注射（半规管造口术）已被证明是将药物局部递送至内耳的一种有效方法。本文的目的是详细描述成年和新生小鼠半规管造口术所涉及的手术技术。如通过快绿染料和携带绿色荧光蛋白基因的腺相关病毒血清型8所示，半规管造口术有助于注射试剂在耳蜗和前庭终器中广泛分布，同时对听力和前庭功能的损害最小。该手术在成年和新生小鼠中均成功实施；实际上，如果需要，可以进行多次手术。总之，半规管造口术是一种向成年和新生小鼠内耳递送药物的有效且安全方法，未来可能用于治疗人类内耳疾病。
