Isgrig Kevin, Chien Wade W
National Institute on Deafness and Other Communication Disorders/National Institutes of Health, Bethesda, MD, USA.
Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Methods Mol Biol. 2019;1937:221-226. doi: 10.1007/978-1-4939-9065-8_13.
Inner ear gene therapy offers great potential as a treatment for hearing loss and dizziness. The surgical method used to deliver gene therapy into the inner ear is a critical step in determining the success of inner ear gene therapy. Here we describe two commonly used surgical methods for gene delivery in neonatal mouse inner ear: the round window approach and the posterior semicircular canal approach. Both of these approaches are effective at delivering gene therapy to the neonatal mouse inner ear.
内耳基因治疗作为治疗听力损失和头晕的方法具有巨大潜力。将基因治疗输送到内耳所采用的手术方法是决定内耳基因治疗成功与否的关键步骤。在此，我们描述两种在新生小鼠内耳中常用的基因递送手术方法：圆窗途径和后半规管途径。这两种途径在将基因治疗递送至新生小鼠内耳方面均有效。
