新生小鼠内耳基因递送的手术方法
Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse.
作者信息
Isgrig Kevin, Chien Wade W
机构信息
National Institute on Deafness and Other Communication Disorders/National Institutes of Health, Bethesda, MD, USA.
Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins School of Medicine, Baltimore, MD, USA.
出版信息
Methods Mol Biol. 2019;1937:221-226. doi: 10.1007/978-1-4939-9065-8_13.
Abstract
Inner ear gene therapy offers great potential as a treatment for hearing loss and dizziness. The surgical method used to deliver gene therapy into the inner ear is a critical step in determining the success of inner ear gene therapy. Here we describe two commonly used surgical methods for gene delivery in neonatal mouse inner ear: the round window approach and the posterior semicircular canal approach. Both of these approaches are effective at delivering gene therapy to the neonatal mouse inner ear.
摘要
内耳基因治疗作为治疗听力损失和头晕的方法具有巨大潜力。将基因治疗输送到内耳所采用的手术方法是决定内耳基因治疗成功与否的关键步骤。在此，我们描述两种在新生小鼠内耳中常用的基因递送手术方法：圆窗途径和后半规管途径。这两种途径在将基因治疗递送至新生小鼠内耳方面均有效。
相似文献
1
Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse.新生小鼠内耳基因递送的手术方法
Methods Mol Biol. 2019;1937:221-226. doi: 10.1007/978-1-4939-9065-8_13.
2
Canalostomy is an ideal surgery route for inner ear gene delivery in big animal model.外耳道造口术是大型动物模型中内耳基因递送的理想手术途径。
Acta Otolaryngol. 2019 Nov;139(11):939-947. doi: 10.1080/00016489.2019.1654130. Epub 2019 Sep 5.
3
Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse.新生小鼠内耳基因递送的后半规管途径
J Vis Exp. 2018 Mar 2(133):56648. doi: 10.3791/56648.
4
Refining surgical techniques for efficient posterior semicircular canal gene delivery in the adult mammalian inner ear with minimal hearing loss.优化手术技术，以在成年哺乳动物内耳中实现最小听力损失的高效后半规管基因传递。
Sci Rep. 2021 Sep 22;11(1):18856. doi: 10.1038/s41598-021-98412-y.
5
Surgical method for virally mediated gene delivery to the mouse inner ear through the round window membrane.通过圆窗膜将病毒介导的基因递送至小鼠内耳的手术方法。
J Vis Exp. 2015 Mar 16(97):52187. doi: 10.3791/52187.
6
'Soft reinforcement' of the round window for superior semi-circular canal dehiscence syndrome.针对上半规管裂综合征的圆窗“软性强化”
J Laryngol Otol. 2020 Apr;134(4):366-368. doi: 10.1017/S0022215120000353. Epub 2020 Feb 28.
7
Cochlear gene transfer mediated by adeno-associated virus: Comparison of two surgical approaches.腺相关病毒介导的耳蜗基因转移：两种手术方法的比较。
Laryngoscope. 2015 Nov;125(11):2557-64. doi: 10.1002/lary.25317. Epub 2015 Apr 17.
8
Round window plugging in the treatment of superior semicircular canal dehiscence.圆窗堵塞术治疗外半规管裂
Laryngoscope. 2018 Jun;128(6):1445-1452. doi: 10.1002/lary.26899. Epub 2017 Oct 9.
9
Singular neurectomy update. II. Review of 102 cases.单发性神经切除术的最新进展。II. 102例病例回顾。
Laryngoscope. 1991 Aug;101(8):855-62. doi: 10.1288/00005537-199108000-00009.
10
Acoustic stimulation of the semicircular canals.对半规管的听觉刺激。
Otolaryngol Clin North Am. 1995 Feb;28(1):207-19.
引用本文的文献
1
Exploring AAV-Mediated Gene Therapy for Inner Ear Diseases: from Preclinical Success to Clinical Potential.探索腺相关病毒介导的内耳疾病基因治疗：从临床前成功到临床潜力
Adv Sci (Weinh). 2025 Sep;12(33):e08397. doi: 10.1002/advs.202408397. Epub 2025 Jun 20.
2
The rise of cochlear gene therapy.耳蜗基因治疗的兴起。
Mol Ther. 2025 Jun 4;33(6):2343-2349. doi: 10.1016/j.ymthe.2024.11.012. Epub 2024 Nov 8.
3
In utero adeno-associated virus (AAV)-mediated gene delivery targeting sensory and supporting cells in the embryonic mouse inner ear.胚胎鼠内耳感觉和支持细胞的腺相关病毒（AAV）介导的基因传递。
PLoS One. 2024 Jul 19;19(7):e0305742. doi: 10.1371/journal.pone.0305742. eCollection 2024.
4
Combined AAV-mediated gene replacement therapy improves auditory function in a mouse model of human DFNB42 deafness.联合 AAV 介导的基因替代疗法改善人类 DFNB42 耳聋小鼠模型的听觉功能。
Mol Ther. 2023 Sep 6;31(9):2783-2795. doi: 10.1016/j.ymthe.2023.07.014. Epub 2023 Jul 22.
5
The use of nonhuman primates in studies of noise injury and treatment.在噪声损伤和治疗研究中使用非人类灵长类动物。
J Acoust Soc Am. 2019 Nov;146(5):3770. doi: 10.1121/1.5132709.
6
Genetic Therapies for Hearing Loss: Accomplishments and Remaining Challenges.听力损失的基因治疗：成就与尚存挑战
Neurosci Lett. 2019 Nov 20;713:134527. doi: 10.1016/j.neulet.2019.134527. Epub 2019 Oct 3.
7
Antisense Oligonucleotides for the Treatment of Inner Ear Dysfunction.反义寡核苷酸治疗内耳功能障碍。
Neurotherapeutics. 2019 Apr;16(2):348-359. doi: 10.1007/s13311-019-00729-0.
本文引用的文献
1
Local gene therapy durably restores vestibular function in a mouse model of Usher syndrome type 1G.局部基因治疗可持久恢复 1G 型 Usher 综合征小鼠模型的前庭功能。
Proc Natl Acad Sci U S A. 2017 Sep 5;114(36):9695-9700. doi: 10.1073/pnas.1708894114. Epub 2017 Aug 23.
2
Gene Therapy Restores Balance and Auditory Functions in a Mouse Model of Usher Syndrome.基因疗法可恢复乌舍尔综合征小鼠模型的平衡和听觉功能。
Mol Ther. 2017 Mar 1;25(3):780-791. doi: 10.1016/j.ymthe.2017.01.007. Epub 2017 Feb 21.
3
Gene therapy restores auditory and vestibular function in a mouse model of Usher syndrome type 1c.基因治疗可恢复1c型Usher综合征小鼠模型的听觉和前庭功能。
Nat Biotechnol. 2017 Mar;35(3):264-272. doi: 10.1038/nbt.3801. Epub 2017 Feb 6.
4
RNA Interference Prevents Autosomal-Dominant Hearing Loss.RNA干扰可预防常染色体显性遗传性听力损失。
Am J Hum Genet. 2016 Jun 2;98(6):1101-1113. doi: 10.1016/j.ajhg.2016.03.028. Epub 2016 May 26.
5
Tmc gene therapy restores auditory function in deaf mice.Tmc基因疗法可恢复耳聋小鼠的听觉功能。
Sci Transl Med. 2015 Jul 8;7(295):295ra108. doi: 10.1126/scitranslmed.aab1996.
6
Perilymph Kinetics of FITC-Dextran Reveals Homeostasis Dominated by the Cochlear Aqueduct and Cerebrospinal Fluid.异硫氰酸荧光素标记葡聚糖的外淋巴动力学揭示了以内耳导水管和脑脊液为主导的内环境稳定。
J Assoc Res Otolaryngol. 2015 Jun;16(3):357-71. doi: 10.1007/s10162-015-0512-1. Epub 2015 Mar 24.
7
Gene therapy for sensorineural hearing loss.感音神经性听力损失的基因治疗
Ear Hear. 2015 Jan;36(1):1-7. doi: 10.1097/AUD.0000000000000088.
8
Rescue of hearing and vestibular function by antisense oligonucleotides in a mouse model of human deafness.反义寡核苷酸在人类耳聋小鼠模型中对听力和前庭功能的挽救作用。
Nat Med. 2013 Mar;19(3):345-50. doi: 10.1038/nm.3106. Epub 2013 Feb 4.
9
Restoration of hearing in the VGLUT3 knockout mouse using virally mediated gene therapy.利用病毒介导的基因治疗恢复 VGLUT3 敲除小鼠的听力。
Neuron. 2012 Jul 26;75(2):283-93. doi: 10.1016/j.neuron.2012.05.019.
Zotero 插件