Inoue Takafumi
Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku, Tokyo 162-8480, Japan.
Microscopy (Oxf). 2018 Jun 1;67(3):129-143. doi: 10.1093/jmicro/dfy015.
TI Workbench is a software package that serves as a control and analysis center for cellular imaging and electrophysiological experiments. It is unique among general-purpose software packages where it integrates the control of cellular imaging and electrophysiological devices, as well as sophisticated data analyses, which provides superior usability in imaging experiments combined with electrophysiology. During the development over the last 20 years, the range of supported image acquisition devices has expanded from cooled charge-coupled device (CCD) cameras to multi-photon microscope systems. In this review, I outline the concept of TI Workbench together with its unique functions and features derived from ideas emerging during daily experiments in my own lab and in those of my collaborators over the last 20 years. TI Workbench includes standard functions required for time-lapse multicolor fluorescence imaging and electrophysiological experiments, in addition to specialized functions such as random-scan or conventional raster-scan two-photon microscopy packages and fluorescence life time imaging (FLIM) utilities. Data analysis modules, e.g. digital data filters for temporal waveforms of time-lapse image data and electrophysiology and for 2-D image data, and fluorescence correlation spectroscopy (FCS) analysis functions, are well integrated with data acquisition functions. A notebook function holds formatted text, graphs, image and movie data altogether, which are linked to the actual data files. TI Workbench uses Igor Pro software as a back-end output for publishing. In addition, TI Workbench imports several different formats of image and electrophysiology data, serving as a general-purpose data analysis software package.
TI Workbench是一个软件包，用作细胞成像和电生理实验的控制与分析中心。在通用软件包中，它独具特色，集成了细胞成像和电生理设备的控制以及复杂的数据分析功能，在结合电生理的成像实验中提供了卓越的可用性。在过去20年的开发过程中，支持的图像采集设备范围已从冷却电荷耦合器件（CCD）相机扩展到多光子显微镜系统。在这篇综述中，我概述了TI Workbench的概念及其独特功能和特性，这些功能和特性源自过去20年我自己实验室以及我合作者实验室日常实验中产生的想法。TI Workbench除了具备诸如随机扫描或传统光栅扫描双光子显微镜软件包以及荧光寿命成像（FLIM）实用工具等专门功能外，还包括延时多色荧光成像和电生理实验所需的标准功能。数据分析模块，例如用于延时图像数据和电生理的时间波形以及二维图像数据的数字数据滤波器，以及荧光相关光谱（FCS）分析功能，与数据采集功能完美集成。笔记本功能可容纳格式化文本、图表、图像和电影数据，这些数据与实际数据文件相关联。TI Workbench使用Igor Pro软件作为后端输出用于发布。此外，TI Workbench可导入几种不同格式的图像和电生理数据，用作通用数据分析软件包。
