Navarro Marco A, Hibbard Jaime V K, Miller Michael E, Nivin Tyler W, Milescu Lorin S
Division of Biological Sciences, University of Missouri, Columbia, Missouri.
Division of Biological Sciences, University of Missouri, Columbia, Missouri.
Biophys J. 2015 Oct 20;109(8):1521-7. doi: 10.1016/j.bpj.2015.08.045.
Here, we propose two basic concepts that can streamline electrophysiology and imaging experiments in brain slices and enhance data collection and analysis. The first idea is to interface the experiment with a software environment that provides a 3D scene viewer in which the experimental rig, the brain slice, and the recorded data are represented to scale. Within the 3D scene viewer, the user can visualize a live image of the sample and 3D renderings of the recording electrodes with real-time position feedback. Furthermore, the user can control the instruments and visualize their status in real time. The second idea is to integrate multiple types of experimental data into a spatial and temporal map of the brain slice. These data may include low-magnification maps of the entire brain slice, for spatial context, or any other type of high-resolution structural and functional image, together with time-resolved electrical and optical signals. The entire data collection can be visualized within the 3D scene viewer. These concepts can be applied to any other type of experiment in which high-resolution data are recorded within a larger sample at different spatial and temporal coordinates.
在此，我们提出两个基本概念，它们可以简化脑片电生理学和成像实验，并加强数据收集与分析。第一个概念是将实验与一个软件环境相连接，该软件环境提供一个3D场景查看器，其中实验装置、脑片和记录的数据均按比例呈现。在3D场景查看器中，用户可以可视化样本的实时图像以及记录电极的3D渲染图，并获得实时位置反馈。此外，用户可以控制仪器并实时可视化其状态。第二个概念是将多种类型的实验数据整合到脑片的时空图谱中。这些数据可能包括整个脑片的低倍放大图谱以提供空间背景信息，或任何其他类型的高分辨率结构和功能图像，以及时间分辨的电信号和光信号。整个数据收集过程可以在3D场景查看器中可视化。这些概念可应用于任何其他类型的实验，即在较大样本内不同空间和时间坐标处记录高分辨率数据的实验。
