Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering at Nankai University, Tianjin 300071, China.
Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering at Nankai University, Tianjin 300071, China.
J Hazard Mater. 2018 Jun 5;351:330-336. doi: 10.1016/j.jhazmat.2018.03.019. Epub 2018 Mar 12.
Triazole contaminants in water and soil environments can form complexes with metal ions, and therefore affect the bioavailability and toxicity of some heavy metals. In present study, significant increase of copper (Cu) uptake by earthworm (Eisenia fetida) was observed when combined pollution of benzotriazole (BTR) presented in soil. For instance, Cu accumulation in earthworms increased 55% approximately when BTR presented at the BTR/Cu molar ratio of 1:2.5. While the single Cu exposure (at 32 mg kg in soil) resulted in increased malondialdehyde (MDA) content in earthworms from 0.319 to 0.668 nmol mg protein, joint exposure to BTR at BTR/Cu molar ratio of 1:10 significantly decreased the MDA content to 0.405 nmol mg protein. This indicates a potential detoxification effect of BTR to Cu induced oxidative damage in earthworms. Varied Cu subcellular distribution can be observed in earthworms of the single and combined exposure treatments. With the combined exposure of BTR, the proportion of Cu associated with granular fraction, the toxically inert fraction in earthworms, increased from 25% to 39%. This phenomenon can be used to explain the protective effects of BTR against oxidative damage.
唑类污染物在水和土壤环境中可以与金属离子形成配合物，从而影响一些重金属的生物有效性和毒性。在本研究中，当土壤中存在苯并三唑（BTR）的复合污染时，蚯蚓（赤子爱胜蚓）对铜（Cu）的吸收显著增加。例如，当 BTR 与 Cu 的摩尔比为 1:2.5 时，蚯蚓体内 Cu 的积累增加了约 55%。而当单一 Cu 暴露（土壤中 32mg/kg）使蚯蚓体内丙二醛（MDA）含量从 0.319 增加到 0.668 nmol/mg 蛋白时，BTR 与 Cu 的摩尔比为 1:10 的联合暴露则将 MDA 含量降低至 0.405 nmol/mg 蛋白。这表明 BTR 对 Cu 诱导的蚯蚓氧化损伤具有潜在的解毒作用。单一和联合暴露处理的蚯蚓中可以观察到不同的 Cu 亚细胞分布。在 BTR 的联合暴露下，与颗粒部分（蚯蚓中有毒惰性部分）结合的 Cu 的比例从 25%增加到 39%。这种现象可以用来解释 BTR 对氧化损伤的保护作用。
