Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria / Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, 300350, Tianjin, China.
Institute for Future Initiatives, The University of Tokyo, Tokyo, 113-8654, Japan.
Ecotoxicol Environ Saf. 2020 Jul 1;197:110618. doi: 10.1016/j.ecoenv.2020.110618. Epub 2020 Apr 14.
Benzotriazole (BTR) is a common corrosion inhibitor used to protect copper (Cu) and Cu alloys. To reveal the combined subacute toxicity of BTR and Cu at environmental levels on terrestrial animals, the activity of antioxidative enzymes and the glutathione levels in earthworms (Eisenia fetida) of the single or co-exposure treatments were determined. The activity of both antioxidant enzymes and non-enzymatic antioxidants was affected by BTR in earthworms. Moreover, the analyses of lysosomal neutral red retention time and total antioxidant capacity indicated a detoxification effect of BTR on Cu-induced impairments of the antioxidant defense capacity in earthworms. The apoptotic rate of coelomocytes in earthworms of the co-exposure treatment was lower than that in earthworms treated with Cu only, indicating that BTR alleviates Cu mediated lysosomal membrane damage and antioxidant defense system responses in earthworms.
苯并三唑 (BTR) 是一种常见的腐蚀抑制剂，用于保护铜 (Cu) 和铜合金。为了揭示环境水平下 BTR 和 Cu 对陆地动物的联合亚急性毒性，我们测定了单一或共暴露处理后蚯蚓（赤子爱胜蚓）抗氧化酶的活性和谷胱甘肽水平。BTR 会影响蚯蚓中两种抗氧化酶和非酶抗氧化剂的活性。此外，溶酶体中性红保留时间和总抗氧化能力的分析表明，BTR 对 Cu 诱导的蚯蚓抗氧化防御能力损伤具有解毒作用。共暴露处理蚯蚓的体腔细胞凋亡率低于单独用 Cu 处理的蚯蚓，表明 BTR 缓解了 Cu 介导的蚯蚓溶酶体膜损伤和抗氧化防御系统反应。
