基于金纳米颗粒增强靶标的激光解吸/电离质谱法对霉菌的代谢谱分析

Metabolic profiling of moulds with laser desorption/ionization mass spectrometry on gold nanoparticle enhanced target.

作者信息

Arendowski Adrian, Szulc Justyna, Nizioł Joanna, Gutarowska Beata, Ruman Tomasz

机构信息

Faculty of Chemistry, Rzeszów University of Technology, 6 Powstańców Warszawy Ave., 35-959 Rzeszów, Poland.

Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wólczańska 171/173, Łódź 90-924, Poland.

出版信息

Anal Biochem. 2018 May 15;549:45-52. doi: 10.1016/j.ab.2018.03.016. Epub 2018 Mar 16.

DOI:10.1016/j.ab.2018.03.016

PMID:29555326

Abstract

Surface-assisted laser desorption/ionization mass spectrometry on gold nanoparticle enhanced target (AuNPET) technique was used for metabolomic analysis and secondary metabolites detection of two mould strains - Aspergillus versicolor and Penicillium chrysogenum in model conditions on microbiological malt extract agar medium. Results obtained with the use of AuNPET-based mass spectrometry technique were compared with traditional matrix-assisted laser desorption/ionization (MALDI) method based on α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB) matrices. Gold nanoparticle enhanced target method enabled effective ionization of microbial cellular extract ingredients without interference from the matrix and also improved calibration of spectra resulting in the detection of much higher amount of characteristic metabolites for studied organisms than MALDI.

摘要

采用金纳米颗粒增强靶材表面辅助激光解吸/电离质谱（AuNPET）技术，在微生物麦芽提取物琼脂培养基的模型条件下，对两种霉菌菌株——杂色曲霉和产黄青霉进行代谢组学分析和次生代谢产物检测。将基于AuNPET的质谱技术所获得的结果，与基于α-氰基-4-羟基肉桂酸（CHCA）和2,5-二羟基苯甲酸（DHB）基质的传统基质辅助激光解吸/电离（MALDI）方法进行比较。金纳米颗粒增强靶材方法能够有效电离微生物细胞提取物成分，而不受基质干扰，还改善了光谱校准，从而比MALDI检测到更多所研究生物体的特征代谢产物。