Department of Environmental Biotechnology, Lodz University of Technology, Wólczańska 171/173, Łódź 90-924, Poland.
Doctoral School of Engineering and Technical Sciences at the Rzeszow University of Technology, 8 Powstańców Warszawy Ave., 35-959 Rzeszów, Poland.
Toxins (Basel). 2021 Jan 9;13(1):45. doi: 10.3390/toxins13010045.
This study aimed to detect and quantify mycotoxins on building materials using innovative laser mass spectroscopy methods-silver-109/silver/gold nanoparticle-enhanced target surface-assisted laser desorption/ionisation mass spectrometry (AgNPs, AgNPs and AuNPs SALDI). Results from SALDI-type methods were also compared with commonly used matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. Standards of seven moulds mycotoxin in a final concentration of 100 µg/mL for patulin, citrinin, 3-nitropropionic acid, alternariol and 20 µg/mL for sterigmatocystin, cyclopiazonic acid, roquefortine C in the mixture were tested in pure solutions and after extraction from the plasterboards. Among the studied SALDI-type method, the lowest detection limits and the highest signal intensity of the mycotoxins tested were obtained with the use of AgNPs SALDI MS. The AgNPs method may be considered as an alternative to the currently most frequently used method MALDI MS and also liquid chromatography tandem mass spectrometry LC-MS/MS for mycotoxin determination. Future studies should attempt to use these methods for mass spectrometry imaging (MSI) to evaluate spatial distribution and depth of mycotoxin penetration into building materials.
本研究旨在使用创新的激光质谱方法——银-109/银/金纳米颗粒增强靶面辅助激光解吸/电离质谱（AgNPs、AgNPs 和 AuNPs SALDI）检测和定量建筑材料中的霉菌毒素。SALDI 类型方法的结果也与常用的基质辅助激光解吸/电离（MALDI）质谱法进行了比较。对纯溶液中和从石膏板中提取后的终浓度为 100 µg/mL 的七种霉菌毒素标准品（展青霉素、桔青霉素、3-硝基丙酸、交链孢酚和 20 µg/mL 的 曲霉醇和环匹罗司胺、罗可夫丁 C）进行了测试。在所研究的 SALDI 类型方法中，使用 AgNPs SALDI MS 获得了测试霉菌毒素的最低检测限和最高信号强度。AgNPs 方法可以考虑作为目前最常用的 MALDI MS 方法的替代方法，也可以作为用于霉菌毒素测定的液相色谱串联质谱法（LC-MS/MS）的替代方法。未来的研究应尝试将这些方法用于质谱成像（MSI），以评估霉菌毒素在建筑材料中的空间分布和渗透深度。
