确定DarR：一种对D-天冬氨酸有反应的LysR型转录调节因子。
D-fining DarR: a LysR-type transcriptional regulator that responds to D-aspartate.
作者信息
Mandel Mark J
机构信息
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI USA
出版信息
J Bacteriol. 2018 Mar 19;200(15):e00121-18. doi: 10.1128/JB.00121-18.
Abstract
Work from Jones, Stabb, et al. describes a D-aspartate sensing system in Proteobacteria. D-amino acids are critical components of peptidoglycan and other structures. The new study identifies the LysR-type transcriptional regulator, DarR, which activates the aspartate racemase RacD. Overexpression of RacD enables it to synthesize D-glutamate and restore normal peptidoglycan in a mutant. This study contributes to emerging roles for D-amino acids and how they are synthesized under distinct conditions.
摘要
琼斯、斯塔布等人的研究描述了变形菌中的一种D-天冬氨酸传感系统。D-氨基酸是肽聚糖和其他结构的关键组成部分。这项新研究鉴定出了LysR型转录调节因子DarR，它可激活天冬氨酸消旋酶RacD。RacD的过表达使其能够合成D-谷氨酸，并在突变体中恢复正常的肽聚糖。这项研究有助于揭示D-氨基酸的新作用以及它们在不同条件下的合成方式。
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本文引用的文献
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J Bacteriol. 2018 Jul 10;200(15). doi: 10.1128/JB.00773-17. Print 2018 Aug 1.
2
Overexpression of a newly identified d-amino acid transaminase in Mycobacterium smegmatis complements glutamate racemase deletion.在耻垢分枝杆菌中过表达新鉴定的 D-氨基酸转氨酶可补充谷氨酸消旋酶缺失。
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