Programa de Pós-Graduação em Bioquímica e Bioprospecção, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Caixa Postal 354, Campus Capão do Leão, s/n, Pelotas, 96010-900, RS, Brazil.
Programa de Pós-Graduação em Biociências, Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, 90050-170, RS, Brazil.
Cell Mol Neurobiol. 2018 Jul;38(5):1107-1121. doi: 10.1007/s10571-018-0581-x. Epub 2018 Mar 19.
Altered astrocytic function is a contributing factor to the development of neurological diseases and neurodegeneration. Berry fruits exert neuroprotective effects by modulating pathways involved in inflammation, neurotransmission, and oxidative stress. The aim of this study was to examine the effects of the lingonberry extract on cellular viability and oxidative stress in astrocytes exposed to lipopolysaccharide (LPS). In the reversal protocol, primary astrocytic cultures were first exposed to 1 µg/mL LPS for 3 h and subsequently treated with lingonberry extract (10, 30, 50, and 100 μg/mL) for 24 and 48 h. In the prevention protocol, exposure to the lingonberry extract was performed before treatment with LPS. In both reversal and prevention protocols, the lingonberry extracts, from 10 to 100 μg/mL, attenuated LPS-induced increase in reactive oxygen species (around 55 and 45%, respectively, P < 0.01), nitrite levels (around 50 and 45%, respectively, P < 0.05), and acetylcholinesterase activity (around 45 and 60%, respectively, P < 0.05) in astrocytic cultures at 24 and 48 h. Also, in both reversal and prevention protocols, the lingonberry extract also prevented and reversed the LPS-induced decreased cellular viability (around 45 and 90%, respectively, P < 0.05), thiol content (around 55 and 70%, respectively, P < 0.05), and superoxide dismutase activity (around 50 and 145%, respectively, P < 0.05), in astrocytes at both 24 and 48 h. Our findings suggested that the lingonberry extract exerted a glioprotective effect through an anti-oxidative mechanism against LPS-induced astrocytic damage.
星形胶质细胞功能改变是神经退行性疾病发生和发展的一个重要因素。越橘果通过调节炎症、神经递质传递和氧化应激相关通路发挥神经保护作用。本研究旨在探讨越橘提取物对脂多糖（LPS）作用下星形胶质细胞活力和氧化应激的影响。在逆转实验中，原代星形胶质细胞培养物首先用 1μg/mL LPS 孵育 3 h，然后用越橘提取物（10、30、50 和 100 μg/mL）孵育 24 和 48 h。在预防实验中，先给予越橘提取物，再用 LPS 处理。在逆转和预防实验中，10-100μg/mL 的越橘提取物均可减弱 LPS 诱导的星形胶质细胞中活性氧（分别约 55%和 45%，P<0.01）、亚硝酸盐水平（分别约 50%和 45%，P<0.05）和乙酰胆碱酯酶活性（分别约 45%和 60%，P<0.05）增加，在 24 和 48 h。此外，在逆转和预防实验中，越橘提取物还可预防和逆转 LPS 诱导的星形胶质细胞活力（分别约 45%和 90%，P<0.05）、巯基含量（分别约 55%和 70%，P<0.05）和超氧化物歧化酶活性（分别约 50%和 145%，P<0.05）降低，在 24 和 48 h。我们的研究结果表明，越橘提取物通过抗氧化机制发挥神经保护作用，对抗 LPS 诱导的星形胶质细胞损伤。
