Programa de Pós-Graduação Em Bioquímica E Bioprospecção, Centro de Ciências Químicas, Farmacêuticas E de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, CEP 96010-900, Brazil.
Programa de Pós-Graduação Em Biotecnologia E Biociências, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Neurochem Res. 2021 May;46(5):1129-1140. doi: 10.1007/s11064-021-03248-7. Epub 2021 Feb 5.
Neuroinflammation is an event that occurs in several pathologies of brain. Rubus sp. (blackberry) is a powerful antioxidant fruit, and its extract has neuroprotective activity. The aim of this study was to investigate the blackberry extract properties on lipopolysaccharide (LPS)-induced neuroinflammation, in relation to oxidative parameters and acetylcholinesterase activity in the brain structures of mice. We also investigated interleukin-10 levels in serum. Mice were submitted to Rubus sp. extract treatment once daily for 14 days. On the fifteenth day, LPS was injected in a single dose. LPS induced oxidative brain damage and the blackberry extract demonstrated preventive effects in LPS-challenged mice. LPS administration increased reactive oxygen species levels in the cerebral cortex and striatum, as well as lipid peroxidation in the cerebral cortex. However, the blackberry extract prevented all these parameters. Furthermore, LPS decreased thiol content in the striatum and hippocampus, while a neuroprotective effect of blackberry extract treatment was observed in relation to this parameter. The blackberry extract also prevented a decrease in catalase activity in all the brain structures and of superoxide dismutase in the striatum. An increase in acetylcholinesterase activity was detected in the cerebral cortex in the LPS group, but this activity was decreased in the Rubus sp. extract group. Serum IL-10 levels were reduced by LPS, and the extract was not able to prevent this change. Finally, we observed an antioxidant effect of blackberry extract in LPS-challenged mice suggesting that this anthocyanin-rich extract could be considered as a potential nutritional therapeutic agent for preventive damage associated with neuroinflammation.
神经炎症是发生在多种脑部疾病中的一种事件。悬钩子属（黑莓）是一种强大的抗氧化水果，其提取物具有神经保护活性。本研究旨在研究黑莓提取物对脂多糖（LPS）诱导的神经炎症的特性，以及其对小鼠大脑结构中氧化参数和乙酰胆碱酯酶活性的影响。我们还研究了血清中白细胞介素 10 的水平。小鼠每天接受悬钩子属提取物处理一次，共 14 天。在第 15 天，单次注射 LPS。LPS 诱导了氧化的脑损伤，而黑莓提取物在 LPS 挑战的小鼠中表现出预防作用。LPS 给药增加了大脑皮质和纹状体中的活性氧水平以及大脑皮质中的脂质过氧化。然而，黑莓提取物预防了所有这些参数。此外，LPS 降低了纹状体和海马中的巯基含量，而黑莓提取物的治疗具有神经保护作用。黑莓提取物还预防了所有大脑结构中过氧化氢酶活性的降低和纹状体中超氧化物歧化酶活性的降低。LPS 组大脑皮质中的乙酰胆碱酯酶活性增加，但在悬钩子属提取物组中，该活性降低。LPS 降低了血清中 IL-10 的水平，而提取物未能预防这种变化。最后，我们观察到 LPS 挑战的小鼠中黑莓提取物具有抗氧化作用，这表明这种富含花青素的提取物可被视为与神经炎症相关的预防损伤的潜在营养治疗剂。
