Yuan Yin, Xu Xiu-Yue, Lao Jie, Zhao Xin
Department of Hand Surgery, Huashan Hospital, Fudan University; Key Laboratory of Hand Reconstruction, Ministry of Health; Shanghai Key Laboratory of Peripheral Nerve & Microsurgery, Shanghai, China.
Neural Regen Res. 2018 Feb;13(2):331-339. doi: 10.4103/1673-5374.226429.
Nerve transfer is the most common treatment for total brachial plexus avulsion injury. After nerve transfer, the movement of the injured limb may be activated by certain movements of the healthy limb at the early stage of recovery, i.e., trans-hemispheric reorganization. Previous studies have focused on functional magnetic resonance imaging and changes in brain-derived neurotrophic factor and growth associated protein 43, but there have been no proteomics studies. In this study, we designed a rat model of total brachial plexus avulsion injury involving contralateral C nerve transfer. Isobaric tags for relative and absolute quantitation and western blot assay were then used to screen differentially expressed proteins in bilateral motor cortices. We found that most differentially expressed proteins in both cortices of upper limb were associated with nervous system development and function (including neuron differentiation and development, axonogenesis, and guidance), microtubule and cytoskeleton organization, synapse plasticity, and transmission of nerve impulses. Two key differentially expressed proteins, neurofilament light (NFL) and Thy-1, were identified. In contralateral cortex, the NFL level was upregulated 2 weeks after transfer and downregulated at 1 and 5 months. The Thy-1 level was upregulated from 1 to 5 months. In the affected cortex, the NFL level increased gradually from 1 to 5 months. Western blot results of key differentially expressed proteins were consistent with the proteomic findings. These results indicate that NFL and Thy-1 play an important role in trans-hemispheric organization following total brachial plexus root avulsion and contralateral C nerve transfer.
神经移植是全臂丛神经撕脱伤最常见的治疗方法。神经移植后，在恢复早期，受损肢体的运动可能会被健侧肢体的某些运动激活，即跨半球重组。以往的研究主要集中在功能磁共振成像以及脑源性神经营养因子和生长相关蛋白43的变化，但尚无蛋白质组学研究。在本研究中，我们设计了一种涉及对侧C神经移植的全臂丛神经撕脱伤大鼠模型。然后使用相对和绝对定量的等压标签以及蛋白质免疫印迹分析来筛选双侧运动皮质中差异表达的蛋白质。我们发现上肢双侧皮质中大多数差异表达的蛋白质与神经系统发育和功能（包括神经元分化和发育、轴突发生和导向）、微管和细胞骨架组织、突触可塑性以及神经冲动传递有关。鉴定出两种关键的差异表达蛋白，即神经丝轻链（NFL）和Thy-1。在对侧皮质中，NFL水平在移植后2周上调，在1个月和5个月时下调。Thy-1水平在1至5个月时上调。在患侧皮质中，NFL水平从1至5个月逐渐升高。关键差异表达蛋白的蛋白质免疫印迹结果与蛋白质组学研究结果一致。这些结果表明，NFL和Thy-1在全臂丛神经根撕脱和对侧C神经移植后的跨半球组织中起重要作用。
