López G, Díaz-Cárdenas C, David Alzate J, Gonzalez L N, Shapiro N, Woyke T, Kyrpides N C, Restrepo S, Baena S
Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Pontificia Universidad Javeriana, POB 56710, Bogotá DC, Colombia.
Colombian Center for Genomics and Bioinformatics of Extreme Environments - GeBiX, Bogotá, DC, Colombia.
Int J Syst Evol Microbiol. 2018 May;68(5):1608-1615. doi: 10.1099/ijsem.0.002718. Epub 2018 Mar 20.
Three morphologically similar thermo-acidophilic strains, USBA-GBX-501, USBA-GBX-502 and USBA-GBX-503, were isolated from acidic thermal springs at the National Natural Park Los Nevados (Colombia). All isolates were spore-forming, Gram-stain-positive and motile, growing aerobically at 25-55 °C (optimum ~45 °C) and at pH 1.5-4.5 (optimum pH ~3.0). Phylogenetic analysis of the 16S rRNA gene sequences of these isolates showed an almost identical sequence (99.0 % similarity) and they formed a robust cluster with the closest relative Alicyclobacillus tolerans DSM 16297 with a sequence similarity of 99.0 %. Average similarity to other species of the genus Alicyclobacillus was 93.0 % and average similarity to species of the genus Effusibacillus was 90 %. In addition, the level of DNA-DNA hybridization between strain USBA-GBX-503 and Alicyclobacillus tolerans DSM 16297 was 31.7 %. The genomic DNA G+C content of strain USBA-GBX-503 was 44.6 mol%. The only menaquinone was MK-7 (100.0 %). No ω-alicyclic fatty acids were detected in strain USBA-GBX-503, and the major cellular fatty acids were C18 : 1ω7c, anteiso-C17 : 0 and iso-C17 : 0. Based on phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA-DNA relatedness values, along with low levels of identity at the whole genome level (ANIb and ANIm values of <67.0 and <91.0 %, respectively), it can be concluded that strain USBA-GBX-503 represents a novel species of the genus Alicyclobacillus, for which the name Alicyclobacillus montanus sp. nov. is proposed. The type strain is USBA-GBX-503 (=CMPUJ UGB U503=CBMAI1927).
从哥伦比亚洛斯内瓦多斯国家自然公园的酸性温泉中分离出三株形态相似的嗜热嗜酸菌株，分别为USBA-GBX-501、USBA-GBX-502和USBA-GBX-503。所有分离株均形成芽孢，革兰氏染色阳性且具运动性，在25-55°C（最适温度约45°C）和pH 1.5-4.5（最适pH约3.0）条件下好氧生长。对这些分离株的16S rRNA基因序列进行系统发育分析，结果显示它们的序列几乎相同（相似度为99.0%），并且与亲缘关系最近的耐酸 Alicyclobacillus tolerans DSM 16297 形成了一个稳定的聚类，序列相似度为99.0%。与 Alicyclobacillus 属其他物种的平均相似度为93.0%，与 Effusibacillus 属物种的平均相似度为90%。此外，USBA-GBX-503菌株与耐酸 Alicyclobacillus tolerans DSM 16297之间的DNA-DNA杂交水平为31.7%。USBA-GBX-503菌株的基因组DNA G+C含量为44.6 mol%。唯一的甲基萘醌是MK-7（100.0%）。在USBA-GBX-503菌株中未检测到ω-脂环族脂肪酸，主要的细胞脂肪酸为C18 : 1ω7c、anteiso-C17 : 0和iso-C17 : 0。基于表型和化学分类特征、系统发育分析以及DNA-DNA相关性值，再加上全基因组水平的低同一性（ANIb和ANIm值分别<67.0%和<91.0%），可以得出结论，USBA-GBX-503菌株代表了 Alicyclobacillus 属的一个新物种，为此提出名为 Alicyclobacillus montanus sp. nov. 的新名称。模式菌株为USBA-GBX-503（=CMPUJ UGB U503=CBMAI1927）。
