Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Pontificia Universidad Javeriana, POB 56710, Bogotá DC, Colombia.
Vicepresidency of Research and Creation, Universidad de los Andes, Cra 1 No. 18A-12, Bogotá DC, Colombia.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5888-5898. doi: 10.1099/ijsem.0.004490.
Two morphologically similar halophilic strains, named USBA 874 and USBA 960, were isolated from water and sediment samples collected from the Zipaquirá salt mine in the Colombian Andes. Both isolates had non-spore-forming, Gram-stain-negative and motile cells that grew aerobically. The strains grew optimally at 30 °C, pH 7.0 and with 25 % NaCl (w/v). The isolates showed almost identical 16S rRNA gene sequences (99.0 % similarity). The predominant quinones of USBA-960 were Q-8, Q-7 and Q-9. The major cellular fatty acids were C cyclo 8, C and C. According to 16S rRNA gene sequencing, the closest phylogenetic relatives are species (similarity between 93.6 and 92.3 %), OUC007 (88.6 %) and 22II-S10r2 (88.7 %). In addition, the result of genome blast distance phylogeny analysis between strains USBA 874 and USBA 960, (YIM 95161), (E1L3A), (MK-B5) and (YTM-1) was 18.5 %. Other species delineation analyses also showed low identity such as ANIb and ANIm values (<69.0 and <84.0 % respectively), TETRA (<0.81) and AAI values (<0.67). Genome sequencing of USBA 960 revealed a genome size of 2.47 Mbp and a G+C content of 59.71 mol%. Phylogenetic analysis of strains USBA 874 and USBA 960 indicated that they formed a different lineage within the family . Based on phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA-DNA relatedness values, along with identity at whole genome level, it can be concluded that strains USBA 960 and USBA 874 represent a novel genus of the family and the name gen. nov., sp. nov. is proposed. The type strain is USBA 960 (CMPUJ U095=CECT 30006).
两株形态相似的嗜盐菌株，分别命名为 USBA 874 和 USBA 960，从哥伦比亚安第斯山脉 Zipaquirá 盐矿的水样和沉积物样本中分离得到。两株菌均为非孢子形成、革兰氏阴性、运动型细胞，可在有氧条件下生长。菌株在 30°C、pH7.0 和 25%NaCl（w/v）条件下生长最佳。菌株的 16S rRNA 基因序列几乎完全相同（相似度为 99.0%）。USBA-960 的主要醌类物质为 Q-8、Q-7 和 Q-9。主要的细胞脂肪酸为 C 环 8、C 和 C。根据 16S rRNA 基因测序，最接近的系统发育亲缘关系是 种（相似度在 93.6%至 92.3%之间）、OUC007（88.6%）和 22II-S10r2（88.7%）。此外，菌株 USBA 874 和 USBA 960 之间的全基因组blast 距离系统发育分析结果显示，与 (YIM 95161)、 (E1L3A)、 (MK-B5) 和 (YTM-1) 的相似度为 18.5%。其他 种的划分分析也显示出较低的相似度，如 ANIb 和 ANIm 值（分别<69.0%和<84.0%）、TETRA（<0.81）和 AAI 值（<0.67）。USBA 960 的基因组测序显示其基因组大小为 2.47 Mbp，G+C 含量为 59.71 mol%。菌株 USBA 874 和 USBA 960 的系统发育分析表明，它们在 科内形成了一个不同的谱系。基于表型和化学分类特征、系统发育分析和 DNA-DNA 同源性值，以及全基因组水平的相似度，可以得出结论，菌株 USBA 960 和 USBA 874 代表了 科的一个新属，提议采用新属名 gen. nov.，sp. nov.。模式菌株为 USBA 960（CMPUJ U095=CECT 30006）。
