Błochowiak Katarzyna J, Trzybulska Dorota, Olewicz-Gawlik Anna, Sikora Jan J, Nowak-Gabryel Michalina, Kocięcki Jarosław, Witmanowski Henryk, Sokalski Jerzy
Department of Dental Surgery, Poznan University of Medical Sciences, Poland.
Department of Rheumatology and Clinical Immunology, Poznan University of Medical Sciences, Poland.
Adv Clin Exp Med. 2018 Apr;27(4):455-461. doi: 10.17219/acem/70800.
Aberrant angiogenesis plays a role in the pathogenesis of Sjögren's syndrome (SS).
The aim of this study was to compare the levels of vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) in stimulatory parotid saliva and in serum in healthy subjects (HS), patients with primary SS (pSS) and secondary SS (sSS) and to evaluate the expression of EGF, proangiogenic VEGF165 and antiangiogenic VEGF165 b mRNA isoforms. Additionally, we determined the salivary levels of serine/arginine splicing factor (SRSF1), which regulates VEGF165 and VEGF165 b expression.
The study comprised 34 women (16 with pSS and 18 with sSS) and healthy subjects for blood and saliva sampling. EGF and VEGF levels in saliva and serum and salivary SRSF1 levels were determined by enzyme-linked immunosorbent assay (ELISA). The expression of VEGF165 , VEGF165 b and EGF in peripheral blood mononuclear cells (PBMC) was evaluated by quantitative polymerase chain reaction (qPCR).
There were no differences in the levels of EGF, VEGF, SRSF1 and in the expression of the EGF, VEGF165 and VEGF165 b between HS and SS patients, or between pSS and sSS patients. The salivary levels of VEGF165 and EGF were significantly higher in pSS, sSS and HS than serum levels. Levels of SRSF1 correlated positively with VEGF and EGF levels. Levels of EGF, VEGF and SRSF1 correlated with each other.
The balance of VEGF isoforms is not disturbed in SS. Saliva is more sensitive for the detection of EGF and VEGF than serum, but salivary levels of those proteins are not representative for SS.
异常血管生成在干燥综合征（SS）的发病机制中起作用。
本研究旨在比较健康受试者（HS）、原发性SS（pSS）患者和继发性SS（sSS）患者刺激性腮腺唾液和血清中血管内皮生长因子（VEGF）和表皮生长因子（EGF）的水平，并评估EGF、促血管生成的VEGF165和抗血管生成的VEGF165b mRNA亚型的表达。此外，我们测定了调节VEGF165和VEGF165b表达的丝氨酸/精氨酸剪接因子（SRSF1）的唾液水平。
本研究纳入34名女性（16名pSS患者和18名sSS患者）以及用于采集血液和唾液样本的健康受试者。通过酶联免疫吸附测定（ELISA）法测定唾液和血清中的EGF和VEGF水平以及唾液中的SRSF1水平。通过定量聚合酶链反应（qPCR）评估外周血单个核细胞（PBMC）中VEGF165、VEGF165b和EGF的表达。
HS与SS患者之间，或pSS与sSS患者之间，EGF、VEGF、SRSF1水平以及EGF、VEGF165和VEGF165b的表达均无差异。pSS、sSS和HS患者的唾液中VEGF165和EGF水平显著高于血清水平。SRSF1水平与VEGF和EGF水平呈正相关。EGF、VEGF和SRSF1水平相互关联。
SS患者中VEGF亚型的平衡未受干扰。唾液比血清对EGF和VEGF的检测更敏感，但这些蛋白质的唾液水平不代表SS。
