Department of Chemistry , Indiana University , 800 East Kirkwood Avenue , Bloomington , Indiana 47405 , United States.
X-ray Science Division, Advanced Photon Source , Argonne National Laboratory , 9700 South Cass Avenue , Argonne , Illinois 60439 , United States.
ACS Nano. 2018 Apr 24;12(4):3541-3550. doi: 10.1021/acsnano.8b00528. Epub 2018 Mar 26.
Bottom-up construction of mesoscale materials using biologically derived nanoscale building blocks enables engineering of desired physical properties using green production methods. Virus-like particles (VLPs) are exceptional building blocks due to their monodispersed sizes, geometric shapes, production ease, proteinaceous composition, and our ability to independently functionalize the interior and exterior interfaces. Here a VLP, derived from bacteriophage P22, is used as a building block for the fabrication of a protein macromolecular framework (PMF), a tightly linked 3D network of functional protein cages that exhibit long-range order and catalytic activity. Assembly of PMFs was electrostatically templated, using amine-terminated dendrimers, then locked into place with a ditopic cementing protein that binds to P22. Long-range order is preserved on removal of the dendrimer, leaving a framework material composed completely of protein. Encapsulation of β-glucosidase enzymes inside of P22 VLPs results in formation of stable, condensed-phase materials with high local concentration of enzymes generating catalytically active PMFs.
使用生物衍生的纳米级构建块自下而上构建介观材料，可以使用绿色生产方法来工程设计所需的物理性质。病毒样颗粒 (VLPs) 是特殊的构建块，因为它们具有单分散的尺寸、几何形状、易于生产、蛋白质组成，并且我们能够独立地对内部和外部界面进行功能化。在这里，源自噬菌体 P22 的 VLP 被用作制造蛋白质大分子框架 (PMF) 的构建块，PMF 是一种紧密连接的功能性蛋白质笼的 3D 网络，具有长程有序和催化活性。使用末端为胺的树枝状大分子通过静电模板组装 PMF，然后用结合到 P22 的双位胶结蛋白将其锁定到位。在去除树枝状大分子后保留长程有序，留下完全由蛋白质组成的框架材料。将β-葡萄糖苷酶酶封装在 P22 VLP 内会形成稳定的凝聚相材料，其中酶的局部浓度很高，生成具有催化活性的 PMF。
