蛋白质大分子框架（PMFs）的制备及其在催化材料中的应用。
Fabrication of Protein Macromolecular Frameworks (PMFs) and Their Application in Catalytic Materials.
机构信息
Department of Chemistry and Biochemistry, California State University, Fresno, Fresno, CA, USA.
Department of Chemistry, Indiana University, Bloomington, IN, USA.
出版信息
Methods Mol Biol. 2023;2671:111-120. doi: 10.1007/978-1-0716-3222-2_6.
The construction of three-dimensional (3D) array materials from nanoscale building blocks has drawn significant interest because of their potential to exhibit collective properties and functions arising from the interactions between individual building blocks. Protein cages such as virus-like particles (VLPs) have distinct advantages as building blocks for higher-order assemblies because they are extremely homogeneous in size and can be engineered with new functionalities by chemical and/or genetic modification. In this chapter, we describe a protocol for constructing a new class of protein-based superlattices, called protein macromolecular frameworks (PMFs). We also describe an exemplary method to evaluate the catalytic activity of enzyme-enclosed PMFs, which exhibit enhanced catalytic activity due to the preferential partitioning of charged substrates into the PMF.
三维（3D）阵列材料由纳米级构建块构建引起了极大的兴趣，因为它们有可能表现出由于个体构建块之间的相互作用而产生的集体性质和功能。蛋白质笼如病毒样颗粒（VLPs）作为高级组装的构建块具有明显的优势，因为它们在尺寸上非常均匀，并且可以通过化学和/或遗传修饰赋予新的功能。在本章中，我们描述了一种构建称为蛋白质大分子框架（PMFs）的新型蛋白质超晶格的方案。我们还描述了一种评估包埋酶的 PMF 的催化活性的示例方法，由于带电荷的底物优先分配到 PMF 中，因此包埋酶的 PMF 表现出增强的催化活性。
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