Isler C M, Bellamy J E, Wobeser G A
Can J Vet Res. 1987 Apr;51(2):271-6.
Reported are the results of preliminary attempts to characterize the molecular weight, heat sensitivity and other features of a labile neurotoxin identified in the serum of calves exhibiting neurological signs in association with coccidial enteritis. The labile neurotoxin activity is heat labile (60 degrees C for 30 min) and is lost upon exposure to acidic pH (5.5) and cysteine (1.75 g/100 mL serum). Activity can be recovered from the precipitate of a 30% wt/vol solution of (NH4)2SO4 in serum. Ultrafiltration trials suggest that labile neurotoxin activity may be linked to a molecule of over 300,000 MW.
报告了对一种不稳定神经毒素的分子量、热敏感性和其他特征进行初步表征的尝试结果。该不稳定神经毒素存在于患有球虫性肠炎并伴有神经症状的犊牛血清中。不稳定神经毒素活性对热不稳定（60℃ 30分钟），暴露于酸性pH值（5.5）和半胱氨酸（1.75 g/100 mL血清）时会丧失。活性可从血清中30%（重量/体积）硫酸铵溶液的沉淀物中恢复。超滤试验表明，不稳定神经毒素活性可能与分子量超过300,000的分子有关。
