International Research Center in Critical Raw Materials-ICCRAM , Universidad de Burgos , Plaza Misael Bañuelos s/n , 09001 Burgos , Spain.
Departamento de Química, Facultad de Ciencias , Universidad de Burgos , 09001 , Burgos , Spain.
ACS Appl Mater Interfaces. 2018 Apr 11;10(14):11467-11473. doi: 10.1021/acsami.7b19440. Epub 2018 Mar 30.
Electrospun biodegradable polymers have emerged as promising materials for their applications in several fields, including biomedicine and food industry. For this reason, the susceptibility of these materials to be colonized by different pathogens is a critical issue for public health, and their study can provide future knowledge to develop new strategies against bacterial infections. In this work, the ability of three pathogenic bacterial species ( Pseudomonas aeruginosa, Acinetobacter baumannii, and Listeria monocytogenes) to adhere and form biofilm in electrospun polycaprolactone (PCL) microfibrous meshes was investigated. Bacterial attachment was analyzed in meshes with different microstructure, and comparisons with other materials (borosilicate glass and electrospun polylactic acid (PLA)) fibers were assessed. Analysis included colony forming unit (CFU) counts, scanning electron microscopy (SEM), and crystal violet (CV) staining. All the obtained data suggest that PCL meshes, regardless of their microstructure, are highly susceptible to be colonized by the pathogenic relevant bacteria used in this study, so a pretreatment or a functionalization with compounds that present some antimicrobial activity or antibiofilm properties is highly recommended before their application. Moreover, an experiment designed to simulate a chronic wound environment was used to demonstrate the ability of these meshes to detach biofilms from the substratum where they have developed, thus making them promising candidates to be used in wound cleaning and disinfection.
电纺可生物降解聚合物因其在生物医学和食品工业等多个领域的应用而成为备受关注的材料。鉴于此，这些材料容易被不同的病原体定殖是一个关乎公共健康的关键问题，对其进行研究可为开发新的抗菌感染策略提供未来的知识。在这项工作中，研究了三种致病性细菌（铜绿假单胞菌、鲍曼不动杆菌和单核细胞增生李斯特菌）在电纺聚己内酯（PCL）微纤维网中粘附和形成生物膜的能力。分析了不同微观结构的微网中细菌的附着情况，并与其他材料（硼硅酸盐玻璃和电纺聚乳酸（PLA）纤维）进行了比较。分析包括菌落形成单位（CFU）计数、扫描电子显微镜（SEM）和结晶紫（CV）染色。所有获得的数据表明，PCL 微网无论其微观结构如何，都极易被本研究中使用的相关致病性细菌定殖，因此，在将其应用之前，强烈建议对其进行预处理或用具有一定抗菌活性或抗生物膜特性的化合物进行功能化。此外，还设计了一个模拟慢性伤口环境的实验，以证明这些微网从其生长的基底上脱落生物膜的能力，这使它们成为在伤口清洁和消毒中应用的有前景的候选材料。
