评价与中国汉族人群甲型肝炎和戊型肝炎病毒感染相关的候选基因。
Evaluation of candidate genes associated with hepatitis A and E virus infection in Chinese Han population.
机构信息
Huai'an Forth Hospital, Huai'an, China.
Department of Epidemiology and Statistics, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow University, 199 Renai Road, Suzhou, 215123, China.
出版信息
Virol J. 2018 Mar 20;15(1):47. doi: 10.1186/s12985-018-0962-2.
BACKGROUND
Recent GWAS-associated studies reported that single nucleotide polymorphisms (SNPs) in ABCB1, TGFβ1, XRCC1 genes were associated with hepatitis A virus (HAV) infection, and variants of APOA4 and APOE genes were associated with and hepatitis E virus (HEV) infection in US population. However, the associations of these loci with HAV or HEV infection in Chinese Han population remain unclear.
METHODS
A total of 3082 Chinese Han persons were included in this study. Anti-HAV IgG and anti-HEV IgG were detected by enzyme-linked immunosorbent assay (ELISA). Genotypes in ABCB1, TGFβ1, XRCC1, APOA4 and APOE SNPs were determined by TaqMan MGB technology.
RESULTS
In Chinese Han population, rs1045642 C to T variation in ABCB1 was significantly associated with the decreased risk of HAV infection (P < 0.05). However, the effect direction was different with the previous US study. Rs1001581 A to G variation in XRCC1, which was not identified in US population, was significantly associated with the protection against HAV infection in our samples (P < 0.05). In addition, our results suggested that rs7412 C to T variation in APOE was significantly associated with lower risk of HEV infection in males (adjusted OR < 1.0, P < 0.05) but not in females.
CONCLUSIONS
ABCB1 and XRCC1 genes variants are significantly associated with the protection against HAV infection. Additionally, Chinese Han males with rs7412 C to T variation in APOE gene are less prone to be infected by HEV.
背景
最近的全基因组关联研究报道，ABCB1、TGFβ1、XRCC1 基因中的单核苷酸多态性（SNPs）与甲型肝炎病毒（HAV）感染有关，APOA4 和 APOE 基因的变异与美国人群的戊型肝炎病毒（HEV）感染有关。然而，这些基因座与中国汉族人群 HAV 或 HEV 感染的关联尚不清楚。
方法
本研究共纳入 3082 例中国汉族人。采用酶联免疫吸附试验（ELISA）检测抗-HAV IgG 和抗-HEV IgG。采用 TaqMan MGB 技术检测 ABCB1、TGFβ1、XRCC1、APOA4 和 APOE SNPs 的基因型。
结果
在中国汉族人群中，ABCB1 基因 rs1045642 C 到 T 变异与 HAV 感染风险降低显著相关（P<0.05）。然而，其作用方向与之前的美国研究不同。XRCC1 基因 rs1001581 A 到 G 变异在美国人群中未被识别，与我们样本中 HAV 感染的保护作用显著相关（P<0.05）。此外，我们的结果表明，APOE 基因 rs7412 C 到 T 变异与男性 HEV 感染风险降低显著相关（调整后的 OR<1.0，P<0.05），但在女性中则不相关。
结论
ABCB1 和 XRCC1 基因变异与 HAV 感染的保护作用显著相关。此外，携带 APOE 基因 rs7412 C 到 T 变异的中国汉族男性不易感染 HEV。
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