Toshimori H, Toshimori K, Oura C, Matsuo H
Cell Tissue Res. 1987 Jun;248(3):627-33. doi: 10.1007/BF00216493.
An immunohistochemical study of atrial natriuretic polypeptides was carried out on embryonic, fetal and neonatal rat hearts, using an antiserum raised against alpha-human atrial natriuretic polypeptide (alpha-hANP). Weakly immunoreactive cells were seen in both atrial and ventricular walls at 11 days post coitum (pc). After this stage, the immunoreactive cells became more intensely stained in both atrial and ventricular walls. The immunoreactivity during the prenatal period was stronger in the superficial cell layer beneath the endocardium, than in the deep cell layer of the atrial wall. The cells in the trabecular meshwork also had an apparent, but weak, immunoreactivity, which showed a greater intensity in the left ventricle than in the right one. It is suggested that these immunoreactive cells in the ventricle may differentiate, in situ, into the cells of the impulse-conducting system during the further development of the heart.
利用抗α-人心房利钠多肽（α-hANP）产生的抗血清，对胚胎、胎儿及新生大鼠心脏进行了心房利钠多肽的免疫组织化学研究。在交配后11天（pc），在心房壁和心室壁均可见弱阳性免疫反应细胞。在此阶段之后，心房壁和心室壁中的免疫反应细胞染色变得更强。产前期间，心内膜下浅表细胞层的免疫反应性比心房壁深层细胞层更强。小梁网中的细胞也有明显但较弱的免疫反应性，左心室的免疫反应性强度大于右心室。提示心室中的这些免疫反应细胞在心脏进一步发育过程中可能原位分化为冲动传导系统的细胞。
