Sagnella G A, Buckley M G, Markandu N D, MacGregor G A
Clin Chim Acta. 1987 Jun 30;166(1):37-44. doi: 10.1016/0009-8981(87)90192-6.
A sensitive and specific procedure for the measurement of atrial natriuretic peptide (ANP) in human plasma by radioreceptor assay, using bovine adrenal membranes treated with Triton-X-100, is described. Plasma levels (mean +/- SEM) of ANP in healthy subjects on a normal sodium intake were 8.4 +/- 1.4 pg/ml and could be modified by changes in sodium intake with increases in sodium intake being associated with higher levels. Mean plasma ANP was approximately 2-fold higher in patients with essential hypertension and 4-fold higher in patients with cardiac or renal disease. The values obtained were comparable in magnitude to those obtained by radioimmunoassay and there was a strong correlation (r = 0.94; p less than 0.001) between the values obtained by radioimmuno- and radioreceptor-assay. These results suggest that circulating ANP corresponds to the biologically active peptide and point to an important role of the atrial peptides in the control of sodium balance.
本文描述了一种通过放射受体分析法测量人血浆中的心钠素（ANP）的灵敏且特异的方法，该方法使用经 Triton-X-100 处理的牛肾上腺膜。正常钠摄入量的健康受试者血浆中 ANP 水平（均值±标准误）为 8.4±1.4 pg/ml，并且可因钠摄入量的变化而改变，钠摄入量增加与更高水平相关。原发性高血压患者的血浆 ANP 均值约高 2 倍，心脏或肾脏疾病患者则高 4 倍。所获得的值在大小上与通过放射免疫分析法获得的值相当，并且放射免疫分析法和放射受体分析法所获得的值之间存在强相关性（r = 0.94；p < 0.001）。这些结果表明循环中的 ANP 与生物活性肽相对应，并指出心房肽在钠平衡控制中起重要作用。
