Ruffin R E, Meki M, Alpers J H
Clin Exp Pharmacol Physiol. 1987 Feb;14(2):87-94. doi: 10.1111/j.1440-1681.1987.tb00961.x.
Sixteen stable asthmatics had the protective effects of inhaled fenoterol (200 micrograms) and inhaled ipratropium bromide (60 micrograms) against standardized histamine inhalation tests at 1 h examined in a randomized double blind fashion. There was no significant difference in the baseline forced expired volume in 1 s (FEV1) for the two study days (P greater than 0.05). There was an increase in FEV1 at 1 h on the fenoterol and ipratropium day compared with the fenoterol day (0.26 versus 0.17 l; P less than 0.05). The geometric mean provocative concentration of histamine to cause a 20% fall in FEV1 (PC20) was 6.31 mg/ml after fenoterol and 8.51 mg/ml after fenoterol and ipratropium (P = 0.038). There was no significant relationship between bronchodilator effect of the bronchodilators and the increase in PC20 from pre-study values, r = 0.307 (P = 0.25) for fenoterol alone and r = 0.195 (P = 0.47) for fenoterol and ipratropium. The relationship between pre-study histamine responsiveness and the increase in PC20 caused by the bronchodilators just failed to reach statistical significance, r = -0.441 (P = 0.09) for fenoterol alone and r = -0.47 (P = 0.06) for fenoterol and ipratropium. The study has shown a greater right shift of histamine responsiveness for combined inhaled fenoterol and ipratropium compared with inhaled fenoterol alone in this group of asthmatics.
16名病情稳定的哮喘患者以随机双盲方式接受了吸入非诺特罗（200微克）和吸入异丙托溴铵（60微克）对标准化组胺吸入试验1小时后的保护作用检测。两个研究日的基线1秒用力呼气量（FEV1）无显著差异（P>0.05）。与单独使用非诺特罗的日子相比，在非诺特罗和异丙托溴铵联合使用日的1小时时FEV1有所增加（0.26升对0.17升；P<0.05）。吸入非诺特罗后组胺使FEV1下降20%的几何平均激发浓度（PC20）为6.31毫克/毫升，吸入非诺特罗和异丙托溴铵后为8.51毫克/毫升（P = 0.038）。支气管扩张剂的支气管扩张作用与PC20相对于研究前值的增加之间无显著相关性，单独使用非诺特罗时r = 0.307（P = 0.25），非诺特罗和异丙托溴铵联合使用时r = 0.195（P = 0.47）。研究前组胺反应性与支气管扩张剂引起PC20增加之间的关系刚未达到统计学显著性，单独使用非诺特罗时r = -0.441（P = 0.09），非诺特罗和异丙托溴铵联合使用时r = -0.47（P = 0.06）。该研究表明，在这组哮喘患者中，与单独吸入非诺特罗相比，联合吸入非诺特罗和异丙托溴铵时组胺反应性有更大的右移。
