Kroeger Michael, Karl Horst, Simmler Bernhard, Singer Peter
technet GmbH, Pestalozzistraße 8, D-70563 Stuttgart, Germany.
Max Rubner-Institut, Palmaille 9, D-22767 Hamburg, Germany.
Heliyon. 2018 Mar 6;4(3):e00552. doi: 10.1016/j.heliyon.2018.e00552. eCollection 2018 Mar.
Up to now the visual inspection of mobility of isolated anisakid larvae serves as a measure of viability and possible risk of infection. This paper presents a new method to rule out unreliability - caused by the temporary immobility of the larvae and by the human uncertainty factor of visual observation. By means of a Near infrared (NIR) imaging method, elastic curvature energies and geometric shape parameters were determined from contours, and used as a measure of viability. It was based on the modelling of larvae as a cylindrical membrane system. The interaction between curvatures, contraction of the longitudinal muscles, and inner pressure enabled the derivation of viability from stationary form data. From series of spectrally signed images within a narrow wavelength range, curvature data of the larvae were determined. Possible mobility of larvae was taken into account in statistical error variables. Experiments on individual living larvae, long-term observations of larvae, and comparative studies of the staining method and the VTD measurements of larvae from the tissue of products confirmed the effectiveness of this method. The VTD differentiated clearly between live and dead nematode larvae isolated from marinated, deep-frozen and salted products. The VTD has been proven as excellent method to detect living anisakid nematode larvae in fishery products and is seen as useful tool for fish processing industry and control authorities.
到目前为止，对分离出的异尖线虫幼虫活动情况的目视检查是衡量其活力及可能感染风险的一种方法。本文提出了一种新方法，以排除由幼虫暂时不动和目视观察的人为不确定性因素导致的不可靠性。通过近红外（NIR）成像方法，从轮廓确定弹性曲率能量和几何形状参数，并将其用作活力的度量。它基于将幼虫建模为圆柱形膜系统。曲率之间的相互作用、纵向肌肉的收缩和内部压力使得能够从静态形态数据推导出活力。从窄波长范围内的一系列光谱标记图像中，确定了幼虫的曲率数据。在统计误差变量中考虑了幼虫可能的活动情况。对单个活幼虫的实验、对幼虫的长期观察以及对来自产品组织的幼虫染色方法和活力检测（VTD）测量的比较研究证实了该方法的有效性。VTD能清楚地区分从腌制、速冻和盐渍产品中分离出的活线虫幼虫和死线虫幼虫。VTD已被证明是检测渔业产品中活异尖线虫幼虫的优秀方法，被视为鱼类加工业和监管当局的有用工具。
