万寿菊（菊科）精油对1型L3期异尖线虫幼虫的活性。

Activity of Tagetes minuta Linnaeus (Asteraceae) essential oil against L3 Anisakis larvae type 1.

作者信息

Giarratana Filippo, Muscolino Daniele, Ziino Graziella, Giuffrida Alessandro, Marotta Stefania Maria, Lo Presti Vittorio, Chiofalo Vincenzo, Panebianco Antonio

机构信息

Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy.

出版信息

Asian Pac J Trop Med. 2017 May;10(5):461-465. doi: 10.1016/j.apjtm.2017.05.005. Epub 2017 May 17.

DOI:10.1016/j.apjtm.2017.05.005

PMID:28647183

Abstract

OBJECTIVE

To evaluate in vitro effects of Tagetes minuta L. essential oil (TEO) on L3 Anisakis larvae type 1.

METHODS

In order to evaluate the potential use of Tagetes minuta essential oil against L3 Anisakis larvae three different media were tested: 1) a saline solution (SS); 2) an industrial marinating solution (MS); 3) sunflower seeds oil (SO). For each media and concentrations of TEO (0.1%, 0.5%, 1.0% and 5.0% v/v), 20 parasites were introduced into plastic Petri dishes (diameter 90 mm) and maintained at room temperature. As controls, larvae were maintained without TEO under identical experimental conditions in SS, MS and SO. A total of 900 larvae were tested. The normalized mean viability, LT100, LT50 and the percentage of inactivation at 24 h were calculated.

RESULTS

In vitro tests revealed a complete inactivation of parasites in saline solution after 2 h with 5% and 1% of TEO. In marinating solution, a complete inactivation of parasites was observed after 4 h at all concentrations used. A slower activity for all TEO concentration was reported in SO.

CONCLUSIONS

The results obtained, showing a strong activity against Anisakis larvae, confirm TEO as a larvicidal agent in the treatment of human anisakidosis and in the industrial marinating process.

摘要

目的

评估小花万寿菊精油（TEO）对1型L3期异尖线虫幼虫的体外作用。

方法

为评估小花万寿菊精油对L3期异尖线虫幼虫的潜在用途，测试了三种不同的培养基：1）盐溶液（SS）；2）工业腌制溶液（MS）；3）向日葵籽油（SO）。对于每种培养基和TEO浓度（0.1%、0.5%、1.0%和5.0% v/v），将20条寄生虫放入直径90毫米的塑料培养皿中，并保持在室温下。作为对照，在相同实验条件下，将幼虫置于不含TEO的SS、MS和SO中培养。共测试了900条幼虫。计算标准化平均活力、LT100、LT50以及24小时的失活百分比。