泛癸利酮对大鼠肾上腺体外促甲状腺激素释放激素释放的影响。
Effects of calcium hopantenate on the release of thyrotropin-releasing hormone from the rat adrenal gland in vitro.
作者信息
Mitsuma T, Sun D H, Nogimori T, Ohtake K, Hirooka Y
机构信息
Fourth Department of Internal Medicine, Aichi Medical University, Japan.
出版信息
Horm Metab Res. 1987 Oct;19(10):475-7. doi: 10.1055/s-2007-1011856.
The effects of calcium hopantenate (HOPA), a GABA agonist, on the release of thyrotropin-releasing hormone (TRH) from the rat adrenal gland were studied in vitro. The adrenal glands were incubated in medium 199 with 1.0 mg/ml of bacitracin (pH 7.4) (medium) for 20 min. The amount of TRH release into the medium was measured by radioimmunoassay. The TRH release from the rat adrenal gland was inhibited significantly in a dose-related manner with the addition of HOPA to the medium. HOPA's effects on TRH release from the adrenal gland were blocked with the addition of bicuculline, a GABA receptor inhibitor. The elution profile of methanol-extracted rat adrenal gland TRH was identical to that for synthetic TRH. The findings suggest that HOPA inhibits TRH release from the rat adrenal gland, and that its effects are mediated via the GABA receptor.
在体外研究了γ-氨基丁酸（GABA）激动剂泛酸钙（HOPA）对大鼠肾上腺促甲状腺激素释放激素（TRH）释放的影响。将肾上腺在含有1.0mg/ml杆菌肽（pH 7.4）的199培养基（培养基）中孵育20分钟。通过放射免疫测定法测量释放到培养基中的TRH量。向培养基中添加HOPA后，大鼠肾上腺的TRH释放以剂量相关的方式受到显著抑制。添加GABA受体抑制剂荷包牡丹碱后，HOPA对肾上腺TRH释放的作用被阻断。甲醇提取的大鼠肾上腺TRH的洗脱图谱与合成TRH的洗脱图谱相同。这些发现表明，HOPA抑制大鼠肾上腺的TRH释放，并且其作用是通过GABA受体介导的。
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