Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen 518004, China.
Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
Int J Mol Sci. 2018 Mar 21;19(4):929. doi: 10.3390/ijms19040929.
(Mill.) Swingle (Simaroubaceae) is a deciduous tree widely distributed throughout temperate regions in China, hence suitable for genetic diversity and evolutionary studies. Previous studies in have mainly focused on its biological activities, genetic diversity and genetic structure. However, until now there is no published report regarding genome of this plant species or Simaroubaceae family. Therefore, in this paper, we first characterized complete chloroplast genome sequence. The tree of heaven chloroplast genome was found to be a circular molecule 160,815 base pairs (bp) in size and possess a quadripartite structure. The chloroplast genome contains 113 unique genes of which 79 and 30 are protein coding and transfer RNA (tRNA) genes respectively and also 4 ribosomal RNA genes (rRNA) with overall GC content of 37.6%. Microsatellite marker detection identified A/T mononucleotides as majority SSRs in all the seven analyzed genomes. Repeat analyses of seven Sapindales revealed a total of 49 repeats in , , , , while , , and had a total of 48 repeats. The phylogenetic analysis using protein coding genes revealed that is a sister to and also suggested that Simaroubaceae is a sister to Rutaceae family. The genome information reported here could be further applied for evolution and invasion, population genetics, and molecular studies in this plant species and family.
（米尔。）斯温格尔（苦木科）是一种落叶乔木，广泛分布于中国温带地区，因此适合进行遗传多样性和进化研究。之前的研究主要集中在其生物活性、遗传多样性和遗传结构上。然而，到目前为止，还没有关于该植物物种或苦木科家族基因组的发表报告。因此，在本文中，我们首先对 完整的叶绿体基因组序列进行了特征描述。发现该树种的叶绿体基因组是一个大小为 160815 碱基对（bp）的圆形分子，具有四分体结构。该 叶绿体基因组包含 113 个独特的基因，其中 79 个和 30 个分别是蛋白质编码和转移 RNA（tRNA）基因，还有 4 个核糖体 RNA（rRNA）基因，总 GC 含量为 37.6%。微卫星标记检测表明，在所有 7 个分析的基因组中，A/T 单核苷酸是大多数 SSRs。对 7 个芸香目植物的重复分析显示，在 、 、 、 中共有 49 个重复，而在 、 、 中则有 48 个重复。利用蛋白质编码基因进行的系统发育分析表明， 与 是姐妹关系，同时也表明苦木科与芸香科是姐妹关系。这里报道的基因组信息可以进一步应用于该植物物种和家族的进化和入侵、种群遗传学和分子研究。
