Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2018 Jan 23;19(1):319. doi: 10.3390/ijms19010319.
The family Balsaminaceae, which consists of the economically important genus and the monotypic genus , lacks a reported or published complete chloroplast genome sequence. Therefore, chloroplast genome sequences of the two sister genera are significant to give insight into the phylogenetic position and understanding the evolution of the Balsaminaceae family among the Ericales. In this study, complete chloroplast (cp) genomes of and were characterized and assembled using a high-throughput sequencing method. The complete cp genomes were found to possess the typical quadripartite structure of land plants chloroplast genomes with double-stranded molecules of 154,189 bp () and 152,238 bp () in length. A total of 115 unique genes were identified in both genomes, of which 80 are protein-coding genes, 31 are distinct transfer RNA (tRNA) and four distinct ribosomal RNA (rRNA). Thirty codons, of which 29 had A/T ending codons, revealed relative synonymous codon usage values of >1, whereas those with G/C ending codons displayed values of <1. The simple sequence repeats comprise mostly the mononucleotide repeats A/T in all examined cp genomes. Phylogenetic analysis based on 51 common protein-coding genes indicated that the Balsaminaceae family formed a lineage with Ebenaceae together with all the other Ericales.
双钩叶科，包括经济上重要的属和单种属，缺乏已报道或发表的完整叶绿体基因组序列。因此，这两个姐妹属的叶绿体基因组序列对于深入了解双钩叶科在桃金娘目中的系统发育位置和进化具有重要意义。本研究采用高通量测序方法对和的完整叶绿体（cp）基因组进行了表征和组装。发现完整的 cp 基因组具有陆地植物叶绿体基因组的典型四分体结构，双链分子长度分别为 154189bp()和 152238bp()。在两个基因组中总共鉴定出 115 个独特基因，其中 80 个是蛋白质编码基因，31 个是独特的转移 RNA(tRNA)和 4 个独特的核糖体 RNA(rRNA)。30 个密码子，其中 29 个以 A/T 结尾的密码子，显示出相对同义密码子使用值>1，而那些以 G/C 结尾的密码子显示出值<1。简单序列重复主要由所有被检测的 cp 基因组中的单核苷酸重复 A/T 组成。基于 51 个常见蛋白质编码基因的系统发育分析表明，双钩叶科与柿科一起形成了桃金娘目内的一个谱系。
