Somogyi Viktoria, Peto Katalin, Deak Adam, Tanczos Bence, Nemeth Norbert
Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Hungary.
Biorheology. 2018;54(5-6):127-140. doi: 10.3233/BIR-17148.
Age- and gender-related alterations of hemorheological parameters have not been completely elucidated to date. Experiments on older animals may give valuable information on this issue. However, the majority of rheological studies have been performed in young rodents.
We aimed to investigate the influence of aging and gender on hemorheological parameters in rats.
Coeval male (n=10) and female (n=10) Wistar (Crl:WI) rats were followed-up over 15 months. Blood samples were obtained from the lateral tail vein at 3, 4, 5, 9, 12, 15 and 18 months of age. Hematological parameters, red blood cell deformability (elongation under shear), osmotic gradient deformability and erythrocyte aggregation were tested. Body weight and the estrus cycle (in females) were also examined.
Erythrocyte aggregation showed age- and gender-related variations. Red blood cell deformability was greater in females and gradually decreased over the 15-month period in both genders. Erythrocyte aggregation was greater in male rats at most ages, but did not show consistent changes with age.
The micro-rheological parameters showed age-related alterations with gender differences. The effect of the estrous cycle cannot be excluded in female rats. The results provide reference data for studies of aging in rats and of the mechanism related to age and gender differences in hemorheology.
迄今为止，血液流变学参数与年龄和性别的相关变化尚未完全阐明。对老年动物进行实验可能会提供有关此问题的有价值信息。然而，大多数流变学研究是在年轻啮齿动物中进行的。
我们旨在研究衰老和性别对大鼠血液流变学参数的影响。
对同时期的雄性（n = 10）和雌性（n = 10）Wistar（Crl:WI）大鼠进行了15个月的随访。在3、4、5、9、12、15和18月龄时从大鼠尾静脉外侧采集血样。检测血液学参数、红细胞变形性（剪切力下的伸长）、渗透梯度变形性和红细胞聚集性。还检查了体重和雌性大鼠的发情周期。
红细胞聚集表现出与年龄和性别相关的变化。雌性大鼠的红细胞变形性更大，并且在15个月期间两性的红细胞变形性均逐渐降低。大多数年龄段雄性大鼠的红细胞聚集性更大，但未显示出随年龄的一致变化。
微观流变学参数显示出与年龄相关的变化以及性别差异。雌性大鼠发情周期的影响不能排除。这些结果为大鼠衰老研究以及血液流变学中与年龄和性别差异相关机制的研究提供了参考数据。
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