Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Clin Hemorheol Microcirc. 2019;71(3):325-335. doi: 10.3233/CH-180411.
Arteriovenous fistula (AVF) may affect erythrocytes through many pathways (e.g., mechanical, inflammatory). However, these effects haven't been elucidated completely yet.
To follow-up the hemorheological and the hematological changes in the presence of artificial carotid-jugular fistula in rats.
Female Wistar rats were subjected to sham-operated group (SG, n = 6) and to fistula group (FG, n = 10). Under general anesthesia, the right carotid artery and jugular vein were isolated via a neck incision, and in the FG carotid-jugular fistula was performed by microsurgical techniques. Hematological variables, red blood cell (RBC) deformability and membrane (mechanical) stability parameters were determined before operation and on the 1st and 6th postoperative weeks. Density separated samples ('young' and 'old' RBCs) were also tested.
In FG group hematocrit, RBC and platelet counts increased gradually to reach highly significant level of increment on the 6th postoperative week. RBC deformability significantly was impaired. The membrane stability test showed lower deformability values after applying mechanical shearing. No significant differences were observed between density separated RBC subpopulations.
The presence of arteriovenous fistula may lead to an increment of RBC mass and impairment of RBC deformability. These changes could be one of the pathways through which the fistula influences the microcirculation.
动静脉瘘（AVF）可能通过多种途径（例如机械、炎症）影响红细胞。然而，这些影响尚未完全阐明。
在大鼠颈动静脉人工瘘模型中，随访血流变学和血液学变化。
雌性 Wistar 大鼠分为假手术组（SG，n=6）和瘘管组（FG，n=10）。在全身麻醉下，通过颈部切口分离右侧颈总动脉和颈内静脉，FG 组通过显微外科技术建立颈动静脉瘘。在术前和术后第 1、6 周测定血液学变量、红细胞（RBC）变形性和膜（机械）稳定性参数。还检测了密度分离的样本（“年轻”和“年老”RBC）。
FG 组的红细胞压积、RBC 和血小板计数逐渐增加，术后第 6 周达到显著增高水平。RBC 变形性显著受损。膜稳定性试验显示机械剪切后变形性值较低。密度分离的 RBC 亚群之间无显著差异。
动静脉瘘的存在可能导致 RBC 质量增加和 RBC 变形性受损。这些变化可能是瘘管影响微循环的途径之一。
