Radosinska Jana, Kollarova Marta, Jasenovec Tomas, Radosinska Dominika, Vrbjar Norbert, Balis Peter, Puzserova Angelika
Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 2, 811 08 Bratislava, Slovakia.
Centre of Experimental Medicine, Slovak Academy of Sciences, Dúbravská Cesta 9, 841 04 Bratislava, Slovakia.
Biology (Basel). 2023 Jul 21;12(7):1030. doi: 10.3390/biology12071030.
Erythrocyte deformability, crucial for oxygen delivery to tissues, plays an important role in the etiology of various diseases. As the factor maintaining the erythrocyte deformability, nitric oxide (NO) has been identified. Reduced NO bioavailability also plays a role in the pathogenesis of hypertension. Our aim was to determine whether aging and hypertension affect erythrocyte deformability and NO production by erythrocytes in experimental animals divided into six groups according to age (7, 20 and 52 weeks), labeled WKY-7, WKY-20 and WKY-52 for normotensive Wistar-Kyoto (WKY) rats, and SHR-7, SHR-20 and SHR-52 for spontaneously hypertensive rats (SHR). The filtration method for the determination of erythrocyte deformability and the fluorescent probe DAF-2 DA for NO production were applied. Deformability and NO production by erythrocytes increased at a younger age, while a decrease in both parameters was observed at an older age. Strain-related differences in deformability were observed at 7 and 52 weeks of age. SHR-7 had reduced deformability and SHR-52 had increased deformability compared with age-matched WKY. Changes in NO production under hypertensive conditions are an unlikely primary factor affecting erythrocyte deformability, whereas age-related changes in deformability are at least partially associated with changes in NO production. However, an interpretation of data obtained in erythrocyte parameters observed in SHRs of human hypertension requires precaution.
红细胞变形性对于氧气输送到组织至关重要，在各种疾病的病因学中发挥着重要作用。作为维持红细胞变形性的因素，一氧化氮（NO）已被确定。NO生物利用度降低在高血压的发病机制中也起作用。我们的目的是确定衰老和高血压是否会影响实验动物红细胞的变形性和红细胞产生NO的情况，这些实验动物根据年龄分为六组（7周、20周和52周），正常血压的Wistar-Kyoto（WKY）大鼠标记为WKY-7、WKY-20和WKY-52，自发性高血压大鼠（SHR）标记为SHR-7、SHR-20和SHR-52。应用过滤法测定红细胞变形性，并用荧光探针DAF-2 DA测定NO的产生。红细胞的变形性和NO产生在较年轻时增加，而在较年长时观察到这两个参数均下降。在7周和52周龄时观察到变形性存在品系相关差异。与年龄匹配的WKY相比，SHR-7的变形性降低，SHR-52的变形性增加。高血压条件下NO产生的变化不太可能是影响红细胞变形性的主要因素，而与年龄相关的变形性变化至少部分与NO产生的变化有关。然而，对人类高血压SHR中观察到的红细胞参数数据的解释需要谨慎。
