bla and bla Genes Detection in Isolates of Carbapenem Resistant P. of Hospitalized Patients in Two Hospitals in Iran.

BACKGROUND & OBJECTIVE: Beta-lactam antibiotics resistance specifically Imipenem and Meropenem, the last choices of treatment, causes fatal events in patients with infection. The aim of this study was to detect the VIM and IMP of metallo-beta-lactamase genes in 103 isolates of in two Iranian hospitals.

METHODS

In this study, we evaluated the susceptibility of to a range of β-lactam antibiotics using disk diffusion method as a standard biochemical test. Combined disk test of Imipenem (IMP) and Imipenem plus Ethylenediaminetetraacetic acid (EDTA) was performed as a phenotypic method to find metallo-beta-lactamase producing isolates.Using conventional PCR method; we evaluated VIM and IMP of metallo-beta-lactamase (MBL) genes in 103 isolates of .

RESULTS

Twenty six (25.2%) out of 103 isolates were resistant to Imipenem and 26 (25.2%) to Meropenem. Among 26 Imipenem and Meropenem-resistant strains (25.2%), 19 cases (73.0%) were MBL producing. Using PCR method, we detected the bla and bla genes in 6 (5.8%) and 2(1.9%) of 19 MBL producing isolates, respectively.

CONCLUSIONS

Evaluation of these carbepenemases genes improve epidemiologic researches and also, can be used as a diagnostic tool for discriminating between antibiotics resistant and sensitive strains of as well as follow-up the patients after treatment.