Evaluation of Metallo-β-Lactamase-Production and Carriage of Genes in Isolated from Burn Wound Infections in Isfahan.

BACKGROUND

Metallo-β-lactamase-production among Gram-negative bacteria, including , has become a challenge for treatment of infections due to these resistant bacteria.

OBJECTIVES

The aim of the current study was to evaluate the metallo-β-lactamase-production and carriage of genes among carbapenem-resistant isolated from burn wound infections.

PATIENTS AND METHODS

A cross-sectional study was conducted from September 2014 to July 2015. One hundred and fifty isolates were recovered from 600 patients with burn wound infections treated at Imam-Musa-Kazem Hospital in Isfahan city, Iran. Carbapenem-resistant isolates were screened by disk diffusion using CLSI guidelines. Metallo-β-lactamase-producing isolates were identified using an imipenem-EDTA double disk synergy test (EDTA-IMP DDST). For detection of MBL genes including - and -, polymerase chain reaction (PCR) methods and sequencing were used.

RESULTS

Among the 150 isolates, 144 (96%) were resistant to imipenem by the disk diffusion method, all of which were identified as metallo-β-lactamase-producing isolates by EDTA-IMP DDST. Twenty-seven (18%) and 8 (5.5%) MBL-producing isolates harbored and - genes, respectively.

CONCLUSIONS

Our findings showed a high occurrence of metallo-β-lactamase production among isolates in burn patient infections in our region. Also, there are isolates carrying the - and - genes in Isfahan province.