检测伊朗西北部临床分离的铜绿假单胞菌中产金属β-内酰胺酶基因。
Detection of metallo-β-lactamase-encoding genes among clinical isolates of Pseudomonas aeruginosa in northwest of Iran.
机构信息
Biotechnology Research Center, Tabriz University of Medical Science, Tabriz, Iran.
出版信息
Diagn Microbiol Infect Dis. 2010 Nov;68(3):322-5. doi: 10.1016/j.diagmicrobio.2010.06.018. Epub 2010 Sep 16.
The prevalence of metallo-β-lactamase (MBL) production among 104 clinical isolates of Pseudomonas aeruginosa from northwest of Iran was investigated by phenotypic and polymerase chain reaction (PCR) methods. Thirty-nine (37.50%) of isolates were MBL positive by double-disk synergy test. Results of PCR revealed that 18 (17.31%) and 6 (5.77%) imipenem nonsusceptible isolates of P. aeruginosa carried bla(VIM) and bla(IMP) genes respectively, while 92.4% (62/67) of isolates contained class 1 integron gene. This is the first report of MBL-producing P. aeruginosa from northwest of Iran.
对来自伊朗西北部的 104 株铜绿假单胞菌临床分离株进行了表型和聚合酶链反应（PCR）方法检测金属β-内酰胺酶（MBL）产生情况。双碟协同试验显示 39 株（37.50%）分离株为 MBL 阳性。PCR 结果显示，18 株（17.31%）和 6 株（5.77%）对亚胺培南不敏感的铜绿假单胞菌分离株分别携带 bla(VIM)和 bla(IMP)基因，而 67 株（92.4%）分离株含有 1 类整合子基因。这是伊朗西北部产 MBL 铜绿假单胞菌的首次报告。
Zotero 插件