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微小RNA-454-3p通过靶向c-Met抑制宫颈癌细胞的侵袭和迁移。

MicroRNA-454-3p inhibits cervical cancer cell invasion and migration by targeting c-Met.

作者信息

Guo Yan, Tao Min, Jiang Min

机构信息

Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.

出版信息

Exp Ther Med. 2018 Mar;15(3):2301-2306. doi: 10.3892/etm.2018.5714. Epub 2018 Jan 8.

DOI:10.3892/etm.2018.5714
PMID:29563976
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5854935/
Abstract

Increasing evidence has demonstrated that microRNAs (miRNAs) have a crucial role in the initiation and progression of tumors. The present study aimed to investigate the expression and the role of miRNA-454-3p in human cervical cancer. Human cervical cancer cells were transfected with miRNA-454-3p mimics or negative control miRNA. MTT, Transwell and wound healing assays were performed to investigate the effects of miRNA-454-3p overexpression on cell proliferation, invasion and migration, respectively. The results indicated that miRNA-454-3p was down-regulated in human cervical cancer cell lines, while its ectopic overexpression significantly inhibited their proliferation, migration and invasion. Furthermore, a luciferase reporter assay confirmed that c-met was a novel target of miRNA-454-3p in HeLa cells. In conclusion, the results of the present study suggested that miRNA-454-3p exhibits significant tumor-suppressive effects in cervical cancer by targeting c-met, and may be a potential means of treating cervical cancer.

摘要

越来越多的证据表明，微小RNA（miRNA）在肿瘤的发生和发展中起关键作用。本研究旨在探讨miRNA-454-3p在人宫颈癌中的表达及其作用。用miRNA-454-3p模拟物或阴性对照miRNA转染人宫颈癌细胞。分别进行MTT、Transwell和伤口愈合试验，以研究miRNA-454-3p过表达对细胞增殖、侵袭和迁移的影响。结果表明，miRNA-454-3p在人宫颈癌细胞系中表达下调，而异位过表达则显著抑制其增殖、迁移和侵袭。此外，荧光素酶报告基因检测证实c-met是HeLa细胞中miRNA-454-3p的一个新靶点。总之，本研究结果表明，miRNA-454-3p通过靶向c-met在宫颈癌中表现出显著的肿瘤抑制作用，可能是治疗宫颈癌的一种潜在手段。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/9b4e732d60b8/etm-15-03-2301-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/dfb4f82c16a9/etm-15-03-2301-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/453015a2e85d/etm-15-03-2301-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/1f12f92bba9f/etm-15-03-2301-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/dbae6b8c945e/etm-15-03-2301-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/9b4e732d60b8/etm-15-03-2301-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/dfb4f82c16a9/etm-15-03-2301-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/453015a2e85d/etm-15-03-2301-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/1f12f92bba9f/etm-15-03-2301-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/dbae6b8c945e/etm-15-03-2301-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/5854935/9b4e732d60b8/etm-15-03-2301-g04.jpg

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