Shirmohammadi Khadije, Sohrabi Sareh, Jafarzadeh Samani Zahra, Effatpanah Hosein, Yadegarazari Reza, Saidijam Massoud
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
J Gastrointest Oncol. 2018 Feb;9(1):46-51. doi: 10.21037/jgo.2017.11.04.
The role of microRNAs (miRNAs) in cellular processes such as growth, apoptosis, differentiation and proliferation verifies the importance of miRNAs in carcinogenesis. Moreover, levels of miRNAs are dysregulated in cancer cells, so they could be used as novel classes of biomarkers for diagnosing cancer. The oncogenic role of and its increased expression have been demonstrated in some cancers. In contrast, there is no consensus for expression rate in different cancers. Therefore, this study was done to investigate the role of and in gastric cancer (GC).
The current study was performed on 31 GC tissues as case, and 31 healthy adjacent tissues as a control group. Quantitative reverse transcriptase (q-RT) PCR was used for studying the expression rate of both and .
The expression rate of both miRNAs in cancerous tissues was significantly higher than healthy adjacent tissues (≈10 folds) (P<0.05).
The results showed that the expression rate of both markers was significantly increased in cancerous tissues. Therefore, they can be suggested as potential biomarkers for cancer diagnosis and prognosis as well as targets for therapy.
微小RNA（miRNA）在细胞生长、凋亡、分化和增殖等过程中的作用证实了其在肿瘤发生中的重要性。此外，癌细胞中miRNA水平失调，因此它们可作为诊断癌症的新型生物标志物。 的致癌作用及其表达增加已在某些癌症中得到证实。相比之下，不同癌症中 的表达率尚无共识。因此，本研究旨在探讨 和 在胃癌（GC）中的作用。
本研究以31例GC组织为病例组，31例健康相邻组织为对照组。采用定量逆转录（q-RT）PCR研究 和 的表达率。
两种miRNA在癌组织中的表达率均显著高于健康相邻组织（约10倍）（P<0.05）。
结果表明，两种标志物在癌组织中的表达率均显著升高。因此，它们可作为癌症诊断和预后的潜在生物标志物以及治疗靶点。
