School of Resource and Environment, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, China.
Environ Sci Pollut Res Int. 2018 Jun;25(16):15357-15367. doi: 10.1007/s11356-018-1671-5. Epub 2018 Mar 21.
A hydroponic experiment was performed to investigate the Cd absorption and subcellular distribution in tea plant, Camellia sinensis. Increased Cd accumulation potential was observed in the tea plant in a Cd-enriched environment, but most of the Cd was absorbed by the roots of C. sinensis. The Cd in all the root fractions was mostly distributed in the soluble fraction, followed by the cell wall fraction. By contrast, the Cd was least distributed in the organelle fraction. The adsorption of Cd onto the C. sinensis roots was described well by the Langmuir isotherm model than the Freundlich isotherm. Most of the Cd (38.6 to 59.4%) was integrated with pectates and proteins in the roots and leaves. Fourier transform infrared spectroscopy (FTIR) analysis showed that small molecular organic substances, such as amino acids, organic acids, and carbohydrates with N-H, C=O, C-N, and O-H functional groups in the roots, bonded with Cd(II). The Cd accumulation in the C. sinensis leaves occurred in the cell wall and organelle fractions. C. sinensis has great capability to transport Cd, thereby indicating pollution risk. The metal homeostasis of Fe, Mn, Ca, and Mg in C. sinensis was affected when the Cd concentration was 1.0-15.0 mg/L.
进行了水培实验以研究茶树（Camellia sinensis）对 Cd 的吸收和亚细胞分布。在富镉环境中，观察到茶树的 Cd 积累潜力增加，但 Cd 大部分被 C. sinensis 的根吸收。所有根部分的 Cd 主要分布在可溶部分，其次是细胞壁部分。相比之下，Cd 在细胞器部分的分布最少。Cd 对 C. sinensis 根的吸附可以用 Langmuir 等温线模型很好地描述，而不是 Freundlich 等温线模型。大部分 Cd（38.6%至 59.4%）与根和叶中的果胶和蛋白质结合。傅里叶变换红外光谱（FTIR）分析表明，与 Cd（II）结合的是根中具有 N-H、C=O、C-N 和 O-H 官能团的小分子有机物质，如氨基酸、有机酸和碳水化合物。Cd 在 C. sinensis 叶片中的积累发生在细胞壁和细胞器部分。C. sinensis 具有很强的运输 Cd 的能力，从而表明存在污染风险。当 Cd 浓度为 1.0-15.0 mg/L 时，C. sinensis 中的 Fe、Mn、Ca 和 Mg 的金属内稳态受到影响。
