Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Department of Internal Medicine, Endocrinology Unit, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
J Endocrinol Invest. 2019 Jan;42(1):7-17. doi: 10.1007/s40618-018-0873-5. Epub 2018 Mar 21.
To assess the plasma oxysterol species 7-ketocholesterol (7-Kchol) and cholestane-3β,5α,6β-triol (chol-triol) as biomarkers of oxidative stress in type 1 and type 2 diabetes mellitus (DM).
In total, 26 type 1 and 80 type 2 diabetes patients, along with 205 age- and gender-matched healthy controls, were included in this study. Oxysterols were quantified by liquid chromatography coupled with tandem mass spectrometry and N,N-dimethylglycine derivatization. Correlations between oxysterols and clinical/biochemical characteristics of the diabetes patients, and factors affecting 7-Kchol and chol-triol, were also determined.
Plasma 7-Kchol and chol-triol levels were significantly higher in type 1 and type 2 diabetes patients compared to healthy controls (P < 0.001). Significant positive correlations were observed between oxysterol levels and levels of glycated hemoglobin (HbA1c), glucose, serum total cholesterol, low-density lipoprotein, very-low-density lipoprotein, and triglycerides, as well as the number of coronary risk factors. Statins, oral hypoglycemic agents, and antihypertensive agents reduced the levels of oxysterols in type 2 diabetes patients. Statin use, HbA1c levels, and the number of coronary risk factors accounted for 98.8% of the changes in 7-Kchol levels, and total cholesterol, smoking status, and the number of coronary risk factors accounted for 77.3% of the changes in chol-triol levels in type 2 diabetes patients.
Plasma oxysterol levels in DM, and particularly type 2 DM, may yield complementary information regarding oxidative stress for the clinical follow-up of diabetes patients, especially those with coronary risk factors.
评估血浆氧化固醇物质 7-酮胆固醇（7-Kchol）和胆甾烷-3β，5α，6β-三醇（chol-triol）作为 1 型和 2 型糖尿病（DM）氧化应激的生物标志物。
本研究共纳入 26 例 1 型糖尿病患者和 80 例 2 型糖尿病患者，以及 205 名年龄和性别匹配的健康对照者。采用液相色谱-串联质谱法和 N，N-二甲基甘氨酸衍生化法对氧化固醇进行定量。还确定了氧化固醇与糖尿病患者的临床/生化特征之间的相关性，以及影响 7-Kchol 和 chol-triol 的因素。
1 型和 2 型糖尿病患者的血浆 7-Kchol 和 chol-triol 水平明显高于健康对照组（P<0.001）。氧化固醇水平与糖化血红蛋白（HbA1c）、血糖、血清总胆固醇、低密度脂蛋白、极低密度脂蛋白和甘油三酯水平以及冠状动脉危险因素的数量呈显著正相关。他汀类药物、口服降糖药和降压药降低了 2 型糖尿病患者的氧化固醇水平。他汀类药物的使用、HbA1c 水平和冠状动脉危险因素的数量解释了 7-Kchol 水平变化的 98.8%，而总胆固醇、吸烟状况和冠状动脉危险因素的数量解释了 chol-triol 水平变化的 77.3%在 2 型糖尿病患者中。
DM，特别是 2 型 DM 患者的血浆氧化固醇水平可能为糖尿病患者的临床随访提供有关氧化应激的补充信息，尤其是那些有冠状动脉危险因素的患者。
