Zanjani Behnoush Nasr, Samadi Afshin, Isikhan Selen Yilmaz, Lay Incilay, Beyaz Sengul, Gelincik Asli, Buyukozturk Suna, Arda Nazli
Division of Molecular Biology and Genetics, Institute of Graduate Studies in Sciences, Istanbul University, Istanbul, Turkey.
Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
J Asthma. 2023 Feb;60(2):288-297. doi: 10.1080/02770903.2022.2045310. Epub 2022 Mar 7.
The prevalence of allergic asthma is increasing on a global scale, reflecting changes in air pollution, climatic changes, and other environmental stimulants. In allergic conditions, oxidative stress occurs as a result of immune system activation. Oxidation of cholesterol leads to the formation of oxysterols. The main purpose of the study was to compare plasma levels of two oxysterols, namely 7-ketocholesterol (7-KC) and cholestane-3β, 5α, 6β-triol (C-triol), and a lipid peroxidation product, malondialdehyde (MDA) in allergic asthma patients with those of healthy controls, in order to provide information about the involvement of lipid peroxidation in allergic asthma. Oxysterols were quantified by LC-MS/MS in plasma samples of 120 asthma patients (90 females + 30 males) and 120 healthy controls (matched by age and sex). Plasma MDA level was analyzed by a spectrophotometric method. Plasma 7-KC (39.45 ± 20.37 ng/mL) and C-triol (25.61 ± 10.13 ng/mL) levels in patients were significantly higher than in healthy subjects (17.84 ± 4.26 ng/mL and 10.00 ± 3.90 ng/mL, respectively) ( < 0.001). Plasma MDA levels were also higher in asthmatic patients (4.98 ± 1.77 nmol/mL) than in healthy controls (1.14 ± 0.31 nmol/mL) ( < 0.001). All data support that lipid peroxidation products are involved in allergic asthma. Oxysterols were quantified for the first time in allergic asthma. Since the high plasma 7-KC and C-triol levels of allergic asthma patients correlate with high IgE levels, detection of these oxysterols by LC-MS/MS may be helpful in the clinical monitoring of allergic asthma. Current data may also lead to new approaches for the prevention, diagnosis, and treatment of the disease. Supplemental data for this article is available online at at.
过敏性哮喘的患病率在全球范围内呈上升趋势，这反映了空气污染、气候变化及其他环境刺激因素的变化。在过敏性疾病中，氧化应激是免疫系统激活的结果。胆固醇氧化会导致氧甾醇的形成。本研究的主要目的是比较过敏性哮喘患者与健康对照者血浆中两种氧甾醇（即7-酮胆固醇（7-KC）和胆甾烷-3β,5α,6β-三醇（C-三醇））以及脂质过氧化产物丙二醛（MDA）的水平，以便提供脂质过氧化在过敏性哮喘中所起作用的相关信息。通过液相色谱-串联质谱法（LC-MS/MS）对120例哮喘患者（90例女性 + 30例男性）和120例健康对照者（按年龄和性别匹配）的血浆样本中的氧甾醇进行定量。采用分光光度法分析血浆MDA水平。患者血浆中7-KC（39.45±20.37 ng/mL）和C-三醇（25.61±10.13 ng/mL）水平显著高于健康受试者（分别为17.84±4.26 ng/mL和10.00±3.90 ng/mL）（<0.001）。哮喘患者的血浆MDA水平（4.98±1.77 nmol/mL）也高于健康对照者（1.14±0.31 nmol/mL）（<0.001）。所有数据均支持脂质过氧化产物与过敏性哮喘有关。首次在过敏性哮喘中对氧甾醇进行了定量。由于过敏性哮喘患者血浆中高水平的7-KC和C-三醇与高IgE水平相关，通过LC-MS/MS检测这些氧甾醇可能有助于过敏性哮喘的临床监测。目前的数据也可能为该疾病的预防、诊断和治疗带来新方法。本文的补充数据可在网上获取。
