Department of Physiology, School of Medicine, University of Murcia, Biomedical Research Institute in Murcia (IMIB-Arrixaca), Murcia, Spain.
PLoS One. 2020 Jun 30;15(6):e0234861. doi: 10.1371/journal.pone.0234861. eCollection 2020.
To examine whether the cardiac, renal and uterine physiological hemodynamic changes during gestation are altered in rats with an early and prolonged exposure to a high fat diet (HFD).
Arterial pressure and cardiac, renal, uterine and radial arteries hemodynamic changes during gestation were examined in adult SD rats exposed to normal (13%) (n = 8) or high (60%) (n = 8) fat diets from weaning. Plethysmography, high-resolution high-frequency ultrasonography and clearance of an inulin analog were used to evaluate the arterial pressure and hemodynamic changes before and at days 7, 14 and 19 of gestation.
Arterial pressure was higher (P<0.05) in rats with high than in those with normal (NFD) fat diet before pregnancy (123 ±3 and 110 ±3 mmHg, respectively) and only decreased at day 14 of gestation in rats with NFD (98±4 mmHg, P<0.05). A significant increment in stroke volume (42 ±10%) and cardiac output (51 ±12%) was found at day 19 of pregnancy in rats with NFD. The changes in stroke volume and cardiac output were similar in rats with NFD and HFD. When compared to the values obtained before pregnancy, a transitory elevation in renal blood flow was found at day 14 of pregnancy in both groups. However, glomerular filtration rate only increased (P<0.05) in rats with NFD at days 14 (20 ±7%) and 19 (27 ±8%) of gestation. The significant elevations of mean velocity, and velocity time integral throughout gestation in radial (127 ±26% and 111 ±23%, respectively) and uterine (91 ±16% and 111 ±25%, respectively) arteries of rats with NFD were not found in rats with an early and prolonged HFD.
This study reports novel findings showing that the early and prolonged exposure to a HFD leads to a significant impairment in the renal, uterine and radial arteries hemodynamic changes associated to gestation.
研究在高脂肪饮食（HFD）早期和长期暴露下，妊娠期间心脏、肾脏和子宫的生理血液动力学变化是否会发生改变。
对从断奶起就暴露于正常（13%）（n=8）或高脂肪（60%）（n=8）饮食的成年 SD 大鼠进行妊娠期间的动脉压和心、肾、子宫和桡动脉血液动力学变化检测。应用体积描记法、高频超声和菊粉类似物清除率评估妊娠前和妊娠第 7、14 和 19 天的动脉压和血液动力学变化。
与正常脂肪饮食（NFD）组相比，妊娠前高脂肪饮食（HFD）组大鼠的动脉压更高（P<0.05）[分别为 123±3 和 110±3mmHg]，仅在 NFD 组大鼠妊娠第 14 天下降[98±4mmHg，P<0.05]。NFD 组大鼠妊娠第 19 天发现心搏量（42±10%）和心输出量（51±12%）显著增加。与妊娠前相比，NFD 和 HFD 组大鼠的心脏指数和每搏输出量变化相似。与妊娠前相比，两组大鼠在妊娠第 14 天均发现短暂的肾血流量升高。然而，只有 NFD 组大鼠的肾小球滤过率在妊娠第 14 天（20±7%）和第 19 天（27±8%）增加（P<0.05）。与 NFD 组相比，HFD 组大鼠在妊娠期间桡动脉（分别为 127±26%和 111±23%）和子宫动脉（分别为 91±16%和 111±25%）的平均速度和速度时间积分的显著升高并未发现。
本研究报告了新的发现，表明早期和长期暴露于 HFD 会导致与妊娠相关的肾、子宫和桡动脉血液动力学变化显著受损。
