Hamelin C, Alain R, Houde D, Berthiaume L
Biosci Rep. 1982 Jul;2(7):527-33. doi: 10.1007/BF01115252.
The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian cells and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase activity, they did not all contain DNA-recombining activity. A distinct, perhaps novel, enzyme may therefore promote DNA recombination in these cell-free systems.
通过从多种培养的哺乳动物细胞中提取染色体脱氧核糖核蛋白并检测重组DNA结构的形成，研究了拓扑异构酶在DNA重组中的作用。尽管每种粗制的脱氧核糖核蛋白制剂都含有拓扑异构酶活性，但并非所有制剂都含有DNA重组活性。因此，一种独特的、可能是新的酶可能在这些无细胞系统中促进DNA重组。
