Department of Ophthalmology, Yantaishan Hospital, Yantai, China.
Eur Rev Med Pharmacol Sci. 2018 Mar;22(5):1196-1202. doi: 10.26355/eurrev_201803_14458.
The use of adipose-derived stem cells (ADSCs) to cure the optic nerve injury was never shown previously. Here, we implanted purified ADSCs into optic nerve injury of rats.
Male Sprague Dawley (SD) rats were used in this study. The vision degeneration was detected by Flash-visual evoked potential (F-VEP) assay. The expression of Macrophage-1 (Mac-1), myeloid differentiation factor 88 (MyD88), and nuclear transcription factor-κB (NF-κB) were studied by Western blot. The expression of interleukin (IL)-6 and tumor necrosis factor (TNF)-α in the optical nerve lysates were assessed by enzyme-linked immunosorbent assay (ELISA).
We found out that ADSC implantation inhibits the amplitude decrease and latency increase of the P1 wave caused by the optic nerve injury. The expression of the inflammation associated proteins of the toll-like receptor 4 (TLR4) signaling pathway, including Mac-1, MyD88, NF-κB, IL-6, and TNF-α, were inhibited in the ADSC therapy group compared to the control group.
Our results indicated that ADSC implantation can inhibit the inflammation after the optic nerve injury and improve the functional vision impairment. These findings suggested ADSC implantation as a translational therapy method for optic nerve injury in clinics.
脂肪干细胞（ADSCs）用于治疗视神经损伤以前从未被报道过。在此，我们将纯化的 ADSCs 植入大鼠视神经损伤部位。
本研究使用雄性 Sprague Dawley（SD）大鼠。通过闪光视觉诱发电位（F-VEP）检测评估视力下降情况。通过 Western blot 检测巨噬细胞-1（Mac-1）、髓样分化因子 88（MyD88）和核转录因子-κB（NF-κB）的表达。通过酶联免疫吸附试验（ELISA）评估视神经裂解物中白细胞介素（IL）-6 和肿瘤坏死因子（TNF）-α的表达。
我们发现 ADSC 植入抑制了视神经损伤引起的 P1 波振幅降低和潜伏期延长。与对照组相比，ADSC 治疗组 TLR4 信号通路相关炎症蛋白，包括 Mac-1、MyD88、NF-κB、IL-6 和 TNF-α 的表达受到抑制。
我们的结果表明，ADSC 植入可以抑制视神经损伤后的炎症反应，并改善功能视力障碍。这些发现表明 ADSC 植入作为一种临床视神经损伤的转化治疗方法。
