Xiao Xiao-Hu, Yang Meng, Sui Jin-Lei, Qi Ji-Yan, Fang Yong-Jun, Hu Song-Nian, Tang Chao-Rong
Key Lab of Rubber Biology, Ministry of Agriculture & Rubber Research Institute Chinese Academy of Tropical Agricultural Sciences Danzhou Hainan China.
Beijing Institute of Genomics Chinese Academy of Sciences Beijing China.
FEBS Open Bio. 2016 Dec 12;7(1):4-24. doi: 10.1002/2211-5463.12163. eCollection 2017 Jan.
Calcium-dependent protein kinases (CDPKs or CPKs) play important roles in various physiological processes of plants, including growth and development, stress responses and hormone signaling. Although the CDPK gene family has been characterized in several model plants, little is known about this gene family in (the Para rubber tree). Here, we characterize the entire CDPK and CDPK-related kinase (CRK) gene families comprising 30 genes ( to ) and nine genes ( to ). Structure and phylogeny analyses of these and genes demonstrate evolutionary conservation in these gene families across and other plant species. The expression of and genes was investigated via Solexa sequencing in a range of experimental conditions (different tissues, phases of leaf development, ethylene treatment, and various abiotic stresses). The results suggest that and genes are important components in growth, development, and stress responses of . Parallel studies on the and gene families were also extended to five other plant species (, , , and ). The and genes from different plant species that exhibit similar expression patterns tend to cluster together, suggesting a coevolution of gene structure and expression behavior in higher plants. The results serve as a foundation to further functional studies of these gene families in as well as in the whole plant kingdom.
钙依赖蛋白激酶（CDPKs或CPKs）在植物的各种生理过程中发挥重要作用，包括生长发育、应激反应和激素信号传导。尽管CDPK基因家族已在几种模式植物中得到表征，但对于橡胶树中这个基因家族却知之甚少。在这里，我们对整个CDPK和CDPK相关激酶（CRK）基因家族进行了表征，该家族包括30个基因（从 到 ）和9个基因（从 到 ）。对这些 和 基因的结构和系统发育分析表明，这些基因家族在橡胶树和其他植物物种中具有进化保守性。通过Solexa测序在一系列实验条件下（不同组织、叶片发育阶段、乙烯处理和各种非生物胁迫）研究了 和 基因的表达。结果表明， 和 基因是橡胶树生长、发育和应激反应的重要组成部分。对 和 基因家族的平行研究也扩展到了其他五个植物物种（ 、 、 、 和 ）。来自不同植物物种的表现出相似表达模式的 和 基因倾向于聚集在一起，这表明高等植物中基因结构和表达行为的共同进化。这些结果为进一步研究这些基因家族在橡胶树以及整个植物界中的功能奠定了基础。
