1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry , Moscow, Russia .
2 Pirogov Russian National Research Medical University , Moscow, Russia .
Antioxid Redox Signal. 2018 Aug 20;29(6):569-584. doi: 10.1089/ars.2018.7540. Epub 2018 May 15.
Hydrogen peroxide (HO) is a key signaling molecule involved in the regulation of both physiological and pathological cellular processes. Genetically encoded HyPer probes are currently among the most effective approaches for monitoring HO dynamics in various biological systems because they can be easily targeted to specific cells and organelles. Since its development in 2006, HyPer has proved to be a robust and powerful tool in redox biology research. Recent Advances: HyPer probes were used in a variety of models to study the role of HO in various redox processes. HyPer has been increasingly used in the past few years for in vivo studies, which has already led to many important discoveries, for example, that HO plays a key role in the regulation of signaling cascades involved in development and aging, inflammation, regeneration, photosynthetic signaling, and other biological processes.
In this review, we focus on the main achievements in the field of redox biology that have been obtained from in vivo experiments using HyPer probes.
Further in vivo studies of the role of HO largely depend on the development of more suitable versions of HyPer for in vivo models: those having brighter fluorescence and a more stable signal in response to physiological changes in pH. Antioxid. Redox Signal. 29, 569-584.
过氧化氢 (HO) 是一种参与调节生理和病理细胞过程的关键信号分子。目前，基因编码的 HyPer 探针是监测各种生物系统中 HO 动态的最有效方法之一，因为它们可以轻松靶向特定的细胞和细胞器。自 2006 年开发以来，HyPer 已被证明是氧化还原生物学研究中强大而有效的工具。最新进展：HyPer 探针已在多种模型中用于研究 HO 在各种氧化还原过程中的作用。近年来，HyPer 越来越多地用于体内研究，这已经导致了许多重要的发现，例如 HO 在发育和衰老、炎症、再生、光合作用信号等生物过程中涉及的信号级联调节中起着关键作用。
在这篇综述中，我们重点介绍了使用 HyPer 探针进行体内实验在氧化还原生物学领域取得的主要成就。
HO 作用的进一步体内研究在很大程度上取决于为体内模型开发更合适的 HyPer 版本：那些具有更亮的荧光和更稳定的信号，以响应 pH 值的生理变化。抗氧化。氧化还原信号。29,569-584。
