Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Int J Biol Macromol. 2018 Aug;115:762-766. doi: 10.1016/j.ijbiomac.2018.03.087. Epub 2018 Mar 20.
Bacillus subtilis is a Gram positive, aerobic and motile bacterium. Biofilm formation is an important feature of this bacterium which confers resistance to antimicrobial agents. The use of new antimicrobial reagents which eliminate biofilms are important and necessary. In this study, the effect of secondary metabolites (bacteriocin) from Lactobacillus acidophilus ATCC 4356 on Bacillus subtilis BM19 in the presence and absence of HBsu which is involved in the growth of planktonic cells and biofilm formation, is reported. HBsu nucleoprotein plays several roles in different processes of Bacillus subtilis cells such as replication, transcription, cell division, recombination and repair. In this study, for the first time, the effect of HBsu on biofilm formation is presented.
In the absence of HBsu, purified bacteriocin from L. acidophilus ATCC 4356 was more effective in inhibiting growth of B. subtilis BM19 planktonic cells as well as biofilm formation. The presence of HBsu on the other hand led to increased biofilm formation.
枯草芽孢杆菌是一种革兰氏阳性、需氧和能动的细菌。生物膜的形成是该细菌的一个重要特征，使其能够抵抗抗菌药物。使用能够消除生物膜的新型抗菌试剂是重要且必要的。在这项研究中，报告了来自嗜酸乳杆菌 ATCC 4356 的次级代谢产物（细菌素）对枯草芽孢杆菌 BM19 的影响，无论是在参与浮游细胞生长和生物膜形成的 HBsu 存在或不存在的情况下。HBsu 核蛋白在枯草芽孢杆菌细胞的多个过程中发挥着多种作用，如复制、转录、细胞分裂、重组和修复。在这项研究中，首次提出了 HBsu 对生物膜形成的影响。
在没有 HBsu 的情况下，从嗜酸乳杆菌 ATCC 4356 中纯化的细菌素在抑制枯草芽孢杆菌 BM19 浮游细胞生长和生物膜形成方面更有效。另一方面，HBsu 的存在导致生物膜形成增加。
