Islam Md Sadequl, Islam Md Ariful, Khatun Mst Minara, Saha Sukumar, Basir Md Samiul, Hasan Md-Mahmodul
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh.
J Pathog. 2018 Jan 14;2018:9378976. doi: 10.1155/2018/9378976. eCollection 2018.
Brucellosis is endemic in Bangladesh both in humans and in animals. A number of reasons complicate the diagnosis, as bovine brucellosis can be diagnosed by various serological tests. But the tests have a limitation; when the organism remains intracellular, the disease goes into chronic stage and the antibody titres may decline. The present study was conducted for isolation and detection of spp. by polymerase chain reaction (PCR) from seronegative cows. A total of 360 dairy cows from three geographical regions were screened serologically by Rose Bengal Plate Test (RBPT) where 24 samples were serologically positive and the rest of the samples were serologically negative. Among the 24 seropositive individuals, 11 were culture positive and 6 were culture positive from serologically negative dairy cows. The overall seroprevalence of brucellosis in cattle was 6.6% and in disease condition a higher prevalence was recorded in abortion (28.07%) followed by infertility (13.33%). To confirm the spp. in seronegative dairy cattle, the isolates were extracted and PCR was conducted, which produced 905 bp amplicon size of 6 spp. from milk sample. So, for the detection or eradication of brucellosis, a bacteriological test and a PCR technique should be performed with the serological test of milk.
布鲁氏菌病在孟加拉国的人和动物中均为地方病。多种原因使诊断变得复杂，因为牛布鲁氏菌病可通过各种血清学检测进行诊断。但这些检测存在局限性；当病原体处于细胞内时，疾病进入慢性阶段，抗体滴度可能下降。本研究旨在通过聚合酶链反应（PCR）从血清学阴性的奶牛中分离并检测布鲁氏菌属。通过玫瑰红平板试验（RBPT）对来自三个地理区域的360头奶牛进行了血清学筛查，其中24份样本血清学呈阳性，其余样本血清学呈阴性。在24份血清阳性个体中，11份培养阳性，6份从血清学阴性的奶牛中培养阳性。牛布鲁氏菌病的总体血清阳性率为6.6%，在发病情况下，流产的患病率较高（28.07%），其次是不育（13.33%）。为了确认血清学阴性奶牛中的布鲁氏菌属，提取分离株并进行PCR，从牛奶样本中产生了大小为905 bp的布鲁氏菌属扩增子。因此，为了检测或根除布鲁氏菌病，应结合牛奶的血清学检测进行细菌学检测和PCR技术检测。
