Ntivuguruzwa Jean Bosco, Kolo Francis Babaman, Gashururu Richard, Uwibambe Evodie, Musanayire Vestine, Ingabire Angelique, Umurerwa Lydia, Mwikarago Emil Ivan, van Heerden Henriette
Bovine Tuberculosis and Brucellosis Programme, Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Department of Veterinary Medicine, School of Veterinary Medicine, University of Rwanda, Kigali, Rwanda.
Front Vet Sci. 2022 Oct 11;9:1017851. doi: 10.3389/fvets.2022.1017851. eCollection 2022.
Seroprevalence studies showed that brucellosis is prevalent in cattle in Rwanda with no recent study on the characterization of spp. Therefore, this study aimed to characterize spp. in seropositive herds of cattle farmed at the wildlife-livestock-human interface. Whole blood samples ( = 118), milk ( = 41), and vaginal swabs ( = 51) were collected from 64 seropositive herds. All samples ( = 210) were inoculated onto modified Centro de Investigacion y Tecnologia Agroalimentaria (CITA) selective medium. Cultures were analyzed to detect spp. using 16S-23S ribosomal DNA interspacer region (ITS) PCR, the cultures were speciated using AMOS and Bruce-ladder PCR assays. spp. were detected in 16.7% (35/210) of the samples established from the samples using ITS-PCR. The AMOS PCR assay identified mixed and ( = 6), ( = 7), and ( = 1) from cultures from blood samples; mixed and ( = 1) and ( = 4) from cultures from milk samples; mixed and ( = 6), ( = 8), and ( = 1) from cultures from vaginal swabs. Bruce-ladder PCR assay confirmed and cultures. The isolation of spp. was significantly associated with districts, with the Nyagatare district having more isolates than other districts ( = 0.01). This study identified single or mixed infections in cattle samples in Rwanda, which emphasizes the need to improve brucellosis control at the wildlife-livestock-human interface and raise the awareness of cattle keepers, abattoir workers, laboratory personnel, and consumers of cattle products.
血清学患病率研究表明，布氏杆菌病在卢旺达的牛群中普遍存在，但近期没有关于布鲁氏菌属特征的研究。因此，本研究旨在对在野生动物 - 家畜 - 人类界面养殖的血清阳性牛群中的布鲁氏菌属进行特征分析。从64个血清阳性牛群中采集了全血样本（n = 118）、牛奶样本（n = 41）和阴道拭子样本（n = 51）。所有样本（n = 210）均接种于改良的农业食品研究与技术中心（CITA）选择性培养基上。使用16S - 23S核糖体DNA间隔区（ITS）PCR对培养物进行分析以检测布鲁氏菌属，通过AMOS和布鲁氏阶梯PCR检测对培养物进行菌种鉴定。使用ITS - PCR从样本中检测出16.7%（35/210）的样本中存在布鲁氏菌属。AMOS PCR检测从血液样本培养物中鉴定出混合的羊种布鲁氏菌和牛种布鲁氏菌（n = 6）、猪种布鲁氏菌（n = 7）和犬种布鲁氏菌（n = 1）；从牛奶样本培养物中鉴定出混合的羊种布鲁氏菌和牛种布鲁氏菌（n = 1）和猪种布鲁氏菌（n = 4）；从阴道拭子样本培养物中鉴定出混合的羊种布鲁氏菌和牛种布鲁氏菌（n = 6）、猪种布鲁氏菌（n = 8）和犬种布鲁氏菌（n = 1）。布鲁氏阶梯PCR检测确认了羊种布鲁氏菌和牛种布鲁氏菌培养物。布鲁氏菌属的分离与地区显著相关，尼亚加塔雷地区的分离株比其他地区更多（P = 0.01）。本研究在卢旺达的牛样本中鉴定出单一或混合的布鲁氏菌感染，这强调了在野生动物 - 家畜 - 人类界面改善布氏杆菌病控制以及提高养牛户、屠宰场工人、实验室人员和牛产品消费者意识的必要性。
