阿尔及利亚巴特纳地区通过实时聚合酶链反应检测血清阴性奶牛牛奶中的 spp.
Detection of spp. in milk from seronegative cows by real-time polymerase chain reaction in the region of Batna, Algeria.
作者信息
Sabrina Rabehi, Mossadak Hamdi Taha, Bakir Mamache, Asma Meghezzi, Khaoula Boushaba
机构信息
Department of Veterinary Science, Institute of Veterinary and Agronomic Sciences, University of Batna 1, Batna, Algeria.
Research Laboratory HASAQ, High National Veterinary School, Algiers, Algeria.
出版信息
Vet World. 2018 Mar;11(3):363-367. doi: 10.14202/vetworld.2018.363-367. Epub 2018 Mar 26.
AIM
The aim of this study was to detect spp. DNA in milk samples collected from seronegative cows using the real-time polymerase chain reaction (PCR) assay for diagnosis of brucellosis in seronegative dairy cows to prevent transmission of disease to humans and to reduce economic losses in animal production.
MATERIALS AND METHODS
In this study, 65 milk samples were investigated for the detection of spp. The detection of the gene in all samples was done by real-time PCR assay by comparative cycle threshold method.
RESULTS
The results show that of the 65 DNA samples tested, 2 (3.08%) were positive for infection. The mean cyclic threshold values of real-time PCR test were 37.97 and 40.48, indicating a positive reaction.
CONCLUSION
The results of the present study indicated that the real-time PCR appears to offer several advantages over serological tests. For this reason, the real-time PCR should be validated on representative numbers of -infected and free samples before being implemented in routine diagnosis in human and animal brucellosis for controlling this disease.
目的
本研究的目的是使用实时聚合酶链反应（PCR）检测从血清学阴性奶牛采集的牛奶样本中的[具体物种]DNA，以诊断血清学阴性奶牛的布鲁氏菌病，防止疾病传播给人类，并减少动物生产中的经济损失。
材料与方法
在本研究中，对65份牛奶样本进行了[具体物种]检测。通过比较循环阈值法，采用实时PCR检测所有样本中的[具体基因]。
结果
结果显示，在检测的65份DNA样本中，有2份（3.08%）[具体物种]感染呈阳性。[具体物种]实时PCR检测的平均循环阈值分别为37.97和40.48，表明反应呈阳性。
结论
本研究结果表明，实时PCR似乎比血清学检测具有多个优势。因此，在用于人类和动物布鲁氏菌病的常规诊断以控制该疾病之前，应在具有代表性数量的[具体物种]感染和未感染样本上验证实时PCR。
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