2014年刚果民主共和国流行的甲型（H3N2）和甲型（H1N1）pdm09流感病毒的分子分析

Molecular Analysis of Influenza A(H3N2) and A(H1N1)pdm09 Viruses circulating in the Democratic Republic of Congo, 2014.

作者信息

Nkwembe Edith, Cintron Roxana, Sessions Wendy, Kavunga Hugo, Babakazo Pelagie, Manya Léonie, Muyembe Jean Jacques

机构信息

Institut National de recherches Biomédicales, INRB.

Influenza Division, Centers for Disease Control and Prevention, Atlanta, USA.

出版信息

J Harmon Res Med Health Sci. 2016 Dec;3(4):247-264.

PMID:29569652

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5858567/

Abstract

BACKGROUND

Very little is known about influenza viruses circulating in the Democratic Republic of Congo (DRC). We aim to characterize genetically and antigenically Influenza A(H3N2) and A(H1N1)pdm09 viruses circulating in the country.

METHODS

From August to December 2014, specimens were collected from patients with influenza like-illness (ILI) or severe acute respiratory infection (SARI) in various surveillance sites. Specimens were tested using real time reverse transcription polymerase chain reaction (RT-PCR) method for the detection of influenza viruses. Positive influenza samples with a cycle threshold (Ct) <30 were genetically and antigenically characterized.

RESULTS

32 samples tested were found positive to influenza A with Ct <30. At CDC Atlanta, 28 out of 32 samples (88%) were tested positive for influenza A virus, including 26 seasonal influenza A viruses subtype H3N2 and 2 pandemic influenza A viruses subtype H1N1pdm 2009. The majority of influenza A(H3N2) viruses were antigenically related to the vaccine virus, while two influenza A(H1N1)pdm09 isolates were antigenically characterized as -like. All A(H3N2) and A(H1N1)pdm09 virus isolates characterized were sensitive to oseltamivir and zanamivir.

CONCLUSION

Two genetically distinct influenza subtypes were co-circulating in the DRCongo. Effective measures against influenza have been suggested.

摘要

背景

关于刚果民主共和国（DRC）流行的流感病毒，人们了解甚少。我们旨在对该国流行的甲型（H3N2）和甲型（H1N1）pdm09流感病毒进行基因和抗原特性分析。

方法

2014年8月至12月，在各个监测点收集流感样疾病（ILI）或严重急性呼吸道感染（SARI）患者的样本。使用实时逆转录聚合酶链反应（RT-PCR）方法检测样本中的流感病毒。对循环阈值（Ct）<30的流感阳性样本进行基因和抗原特性分析。

结果

32个检测样本被发现对甲型流感病毒呈阳性，Ct<30。在亚特兰大疾病控制与预防中心（CDC），32个样本中有28个（88%）检测出甲型流感病毒呈阳性，其中包括26个季节性甲型H3N2流感病毒和2个甲型H1N1pdm2009大流行性流感病毒。大多数甲型（H3N2）流感病毒在抗原性上与疫苗病毒相关，而两个甲型（H1N1）pdm09分离株在抗原性上被鉴定为类似。所有鉴定的甲型（H3N2）和甲型（H1N1）pdm09病毒分离株对奥司他韦和扎那米韦敏感。